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Edition | Availability |
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1
Immunosorption techniques: fundamentals and applications
1992, Akademie Verlag
in English
- 1st ed.
3055013506 9783055013508
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Book Details
Table of Contents
Fundamental remarks on the immune system
Differentiation of B and T lymphocytes
Antigen recognition and immunoresponse
Antibodies and antigens
Structure of immunoglobulins
Antibody fragments
Antigens and haptens
Antigen-antibody reaction
Antibody generation and propagation
Immunization
Polyclonal antibodies
Monoclonal antibodies
General problems
Solid supports
Activation of supports and immobilization (coupling) of affinity ligands
Activation of supports for covalent fixation
Coupling of ligands
Leakage of ligands (bleeding)
Adsorption and desorption of ligates
Adsorption of ligates
Desorption processes
High performance immunoaffinity chromatography
Avidin/biotin system
Separation and purification of antibodies, haptens and antigens
Purification of antibodies
Pretreatment of antibody source
Non-chromatographic separation procedures
Chromatographic separation procedures
Positive and negative selection of free and structure-bound haptens, antigens and receptors.
(cont) Techniques based on immunosorption
Immunoassays
General considerations
Heterogenous immunoassays
Homogeneous immunoassays
Amplification of immunoassays
Immunoblotting and immunodot techniques
Immunosorbents for therapeutical application: immunosorbent reactors
Immunosensors
Immunosensors with electrochemical detection
Non-electrode sensors.
Edition Notes
Includes bibliographical references (p. 143-166) and index.
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