An edition of Neural stem cells
(2002)
Neural stem cells
methods and protocols
by Tanja Zigova, Paul R. Sanberg, and Juan Raymond Sanchez-Ramos
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Table of Contents
Machine generated contents note: Prefacev
Contributors xi
PART I ISOLATION AND CULTURE OF NSCs
1 Neural Differentiation of Embryonic Stem Cells
K. Sue O'Shea
2 Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens
Eric D. Laywell, Valery G. Kukekov, Oleg Suslov,
Tong Zheng, and Dennis A. Steindler 15
3 Isolation of Stem and Precursor Cells from Fetal Tissue
Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao29
4 Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb
Susan C. Barnett and A. Jane Roskams 41
5 Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium
Edmund Au and A. Jane Roskams49
6 Production of Immortalized Human Neural Crest Stem Cells
Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai,
Myung A. Lee, and Jung H. Bang55
7 Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization
Lamya S. Shihabuddin67
8 Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood
Shijie Song and J. Sanchez-Ramos79
9 Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers
Erin Lavik, Yang D. Teng, Evan Snyder, and Robert Langer 89
PART II CHARACTERIZATION OF NSCs IN VITRO
A. CELLULAR TECHNIQUES
10 Analysis of Cell Generation in the Telencephalic Neuroepithelium
Takao Takahashi, Verne S. Caviness, Jr.,
and Pradeep G. Bhide101
11 Clonal Analyses and Cryopreservation
of Neural Stem Cell Cultures
Angelo L. Vescovi, Rossella Galli, and Angela Gritti115
12 Assessing the Involvement of Telomerase in Stem Cell Biology
Mark P. Mattson, Peisu Zhang, and Weiming Fu 125
13 Detection of Telomerase Activity in Neural Cells
Karen R. Prowse137
14 In Vitro Assays for Neural Stem Cell Differentiation
Marcel M. Daadi 149
15 Electron Microscopy and Lac-Z Labeling
Bela Kosaras and Evan Snyder157
B. ELECTROPHYSIOLOGICAL TECHNIQUES
16 Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells
David S. K. Magnuson and Dante J. Morassutti179
C. MOLECULAR TECHNIQUES
17 Fluorescence In Situ Hybridization
Barbara A. Tate and Rachel L. Ostroff 189
18 RT-PCR Analyses of Differential Gene Expression
in ES-Derived Neural Stem Cells
Theresa E. Gratsch197
19 Differential Display: Isolation of Novel Genes
Theresa E. Gratsch213
20 Cell Labeling and Gene Misexpression by Electroporation
Terence J. Van Raay and Michael R. Stark223
21 Gene Therapy Using Neural Stem Cells
Luciano Conti and Elena Cattaneo 233
22 Modeling Brain Pathologies Using Neural Stem Cells
Simonetta Siplone and Elena Cattaneo245
PART III UTILIZATION/CHARACTERIZATION OF NSCs IN VivO
A. ENDOGENOUS POOLS OF STEM/PROGENITOR CELLS
23 Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model
Marcel M. Daadi 265
24 Identification of Musashil-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods
Yonehiro Kanemura, Shin-ichi Sakakibara,
and Hideyuki Okano273
25 Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo
Sanjay S. P. Magavi and Jeffrey D. Macklis283
26 Immunocytochemical Analysis of Neuronal Differentiation
Sanjay S. P. Magavi and Jeffrey D. Macklis291
27 Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold
Lisa A. Catapano, Sanjay S. P. Magavi,
and Jeffrey D. Macklis299
B. TRANSPLANTATION
28 Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS
Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore307
29 Optimizing Stem Cell Grafting into the CNS
Scott R. Whittemore, Y. Ping Zhang, Christopher B. Shields,
Dante J. Morassutti, and David S. K. Magnuson319
30 Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos
Lorenzo M agrassi327
31 Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells
Tanja Zigova and Mary B. Newman341
32 Routes of Stem Cell Administration in the Adult Rodent
Alison E. Willing, Svitlana Garbuzova-Davis,
Paul R. Sanberg, and Samuel Saporta357
Index3 7 5.
Edition Notes
Includes bibliographical references and index.
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- Created October 7, 2008
- 9 revisions
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| November 15, 2023 | Edited by MARC Bot | import existing book |
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| July 5, 2019 | Edited by MARC Bot | import existing book |
| October 7, 2008 | Created by ImportBot | Imported from Library of Congress MARC record |