Check nearby libraries
Buy this book
![Loading indicator](/images/ajax-loader-bar.gif)
In this thesis, I sought to identify novel components of the "peptide loading complex" (PLC) that is involved in delivering peptide ligands to major histocompatibility complex (MHC) class I molecules. An anti-tapasin affinity column was used for the large-scale isolation of the PLC from EL4 cell microsomes. The isolated PLC was separated on an SDS-PAGE gel and protein components were visualized with silver staining. Proteins of interest were processed for tandem mass spectrometry analysis. GenBank database searching with information obtained from the mass spectrometry analysis revealed three novel components, heat shock cognate protein 70 (Hsc70), ribophorin I and oligosaccharide transferase 48 (OST48). Their presence in the PLC was confirmed by Western blot, suggesting a physical link between protein degradation pathways and the PLC. Based on the findings I discussed how such a link may ensure efficient generation of MHC class I:peptide complexes for T cell surveillance.
Check nearby libraries
Buy this book
![Loading indicator](/images/ajax-loader-bar.gif)
Showing 1 featured edition. View all 1 editions?
Edition | Availability |
---|---|
1
Identification of new components of the MHC class I peptide loading complex.
2006
in English
0494211997 9780494211991
|
aaaa
Libraries near you:
WorldCat
|
Book Details
Edition Notes
Source: Masters Abstracts International, Volume: 45-03, page: 1448.
Thesis (M.Sc.)--University of Toronto, 2006.
Electronic version licensed for access by U. of T. users.
ROBARTS MICROTEXT copy on microfiche.
The Physical Object
ID Numbers
Community Reviews (0)
Feedback?History
- Created October 22, 2008
- 2 revisions
Wikipedia citation
×CloseCopy and paste this code into your Wikipedia page. Need help?
December 15, 2009 | Edited by WorkBot | link works |
October 22, 2008 | Created by ImportBot | Imported from University of Toronto MARC record |