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1
Basic methods in protein purification and analysis: a laboratory manual
2008, Cold Spring Harbor Laboratory Press
in English
0879698683 9780879698683
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Book Details
Table of Contents
Introduction to protein purification strategies
Preparation of cellular and subcellular extracts
One-dimensional polyacrylamide gel electrophoresis
Preparative two-dimensional gel electrophoresis with immobilized PH gradients
Ion-exchange chromatography
Size-exclusion chromatography
Reversed-phase high-performance liquid chromatography
Affinity and immunoaffinity chromatography
Metal chelate affinity chromatography
Multidimensional chromatography of intact proteins
Identification of protein-protein interactions by conventional column chromatography
Identification of associated proteins by coimmunoprecipitation
Chromatin immunoprecipitation of protein complexes
Identification of protein-protein interactions with glutathione-s-transferase fusion proteins
Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis
In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest.
Edition Notes
Includes bibliographical references and index.
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- Created May 11, 2009
- 10 revisions
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