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LEADER: 04711cam a2200481 a 4500
001 011341674-1
005 20080111101926.0
008 070823s2008 nyua bf 001 0 eng
010 $a 2007034892
015 $aGBA771046$2bnb
016 7 $a013833959$2Uk
020 $a9780879697761 (cloth)
020 $a0879697768 (cloth)
020 $a9780879697754 (pbk.)
020 $a087969775X (pbk.)
035 0 $aocn167505068
040 $aDLC$cDLC$dBTCTA$dBAKER$dUKM$dC#P$dYDXCP$dSCB$dDLC
050 00 $aQH506$b.S546 2008
060 00 $a2008 E-262
060 10 $aQH 506$bS617 2008
082 00 $a572.8078$222
245 00 $aSingle-molecule techniques :$ba laboratory manual /$cedited by Paul R. Selvin, Taekjip Ha.
260 $aCold Spring Harbor, N.Y. :$bCold Spring Harbor Laboratory Press,$cc2008.
300 $avii, 507 p. :$bill. (some col.) ;$c29 cm.
504 $aIncludes bibliographical references and index.
505 0 $aThe new era of biology in singulo / T. Ha and P.R. Selvin -- Single-molecule FRET with total internal reflection microscopy / C. Joo and T. Ha -- In vitro and in vivo FIONA and other acronyms for watching molecular motors walk / P.R. Selvin, T. Lougheed, M.T. Hoffman, H. Park, H. Balci, B.H. Blehm, and E. Toprak -- Colocalization of fluorescent probes: Accurate and precise registration with nanometer resolution / L.S. Churchman and J.A. Spudich -- Alternating-laser excitation of single molecules / A.N. Kapanidis, M. Heilemann, E. Margeat, X. Kong, E. Nir, and S. Weiss -- Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy / J.F. Beausang, Y. Sun, M.E. Quinlan, J.N. Forkey, and Y.E. Goldman -- Imaging gene expression in living cells at the single-molecule level / J. Xiao, J. Elf, G.-W. Li, J. Yu, and X.S. Xie -- Single-virus tracking in live cells / M.J. Rust, M. Lakadamyali, B. Brandenburg, and X. Zhuang --
505 0 $aUltrasensitive imaging in live cells using fluorescent quantum dots / S. Courty and M. Dahan -- Imaging real-time gene expression in living systems / A.L. Wells, J.S. Condeelis, R.H. Singer, and D. Zenklusen -- Single-molecule imaging of stochastic signaling events in living cells / S. Matsuoka, Y. Miyanaga, T. Yanagida, and M. Ueda -- Fluorescence correlation spectroscopy in vitro and in vivo / E. Haustein and P. Schwille -- Optical traps to study properties of molecular motors / J.A. Spudich, S.E. Rice, R.S. Rock, T.J. Purcell, and H.M. Warrick -- High-resolution dual-trap optical tweezers with differential detection / C. Bustamante, Y.R. Chemla, and J.R. Moffitt -- Imaging and nanomanipulation of an actomyosin motor / S. Nishikawa, T. Komori, T. Ariga, T. Okada, M. Morimatsu, Y. Ishii, and T. Yanagida -- Single-molecule studies using magnetic traps / T. Lionnet, J.-F. Allemand, A. Revyakin, T.R. Strick, O.A. Saleh, D. Bensimon, and V. Croquette --
505 0 $aProbing polysaccharide and protein mechanics by atomic force microscopy / M. Rabbi and P.E. Marszalek -- Single-molecule analysis of nucleic acids and DNA-protein interactions using nanopores / M. Wanunu and A. Meller -- Single-molecule gold-nanoparticle tracking with high temporal and spatial resolution and without an applied load / A.R. Dunn and J.A. Spudich -- Advances in surface-based assays for single molecules / P.M. Fordyce, M.T. Valentine, and S.M. Block -- Hydrodynamic flow-stretching assay for single-molecule studies of nucleic acid-protein interactions / C.M. Schroeder, P.C. Blainey, S. Kim, and X.S. Xie.
520 $aThis book takes researchers who know nothing about single-molecule analyses to the point where they can successfully design and execute appropriate experiments. The manual will be useful to all who are interested in observing, manipulating, and elucidating the molecular mechanisms and discrete properties of macromolecules. Techniques range from in vivo and in vitro fluorescent-based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores.
650 0 $aMolecular biology$vLaboratory manuals.
650 0 $aBiomolecules$xAnalysis$vLaboratory manuals.
650 0 $aFluorescence microscopy$vLaboratory manuals.
650 0 $aAtomic force microscopy$vLaboratory manuals.
650 0 $aUltrastructure (Biology)$vLaboratory manuals.
650 12 $aMolecular Biology$vLaboratory Manuals.
650 22 $aMicroscopy, Atomic Force$vLaboratory Manuals.
650 22 $aMicroscopy, Fluorescence$vLaboratory Manuals.
650 22 $aMolecular Structure$vLaboratory Manuals.
655 7 $aHandbooks, manuals, etc.$2fast
700 1 $aSelvin, Paul R.
700 1 $aHa, Taekjip.
988 $a20071217
906 $0DLC