MARC Record from harvard_bibliographic_metadata

LEADER: 04173cam a2200385 a 4500
001 012803756-3
005 20111012122435.0
008 110310s2011 nyua a 001 0 eng d
020 $a1617791253
020 $a9781617791253
020 $a9781617791260 (e-isbn)
020 $a1617791261 (e-isbn)
035 0 $aocn706784502
040 $aYDXCP$cYDXCP$dRML
245 00 $aReceptor signal transduction protocols /$cedited by Gary B. Willars and R.A. John Challiss.
260 $aNew York :$bSpringer,$cc2011.
300 $axi, 479 p. :$bill. (some col.) ;$c27 cm.
490 1 $aSpringer protocols
490 1 $aMethods in Molecular Biology ;$v746
504 $aIncludes bibliographical references and index.
530 $aAlso available in an electronic version.
538 $aMode of access: World Wide Web.
505 0 $aInducible expression of G protein-coupled receptors in transfected cells / Beryl Koener and Emmanuel Hermans -- Using the Flp-In T-Rex system to regulate GPCR expression / Richard J. Ward, Elisa Alvarez-Curto, and Graeme Milligan -- Viral infection for GPCR expression in eukaryotic cells / Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi -- Generation of epitope-tagged GPCRs / Yan Huang and Gary B. Willars -- Use of site-directed mutagenesis to study GPCRs / Alex C. Conner ... [et al.] -- Approaches to study GPCR regulation in native systems / Jonathon M. Willets -- Heterologous expression of GPCRs in fission yeast / John Davey and Graham Ladds -- Radioligand binding methods for membrane preparations and intact cells / David B. Bylund and Myron L. Toews -- Quantification of GPCR mRNA using real-time RT-PCR / Trond Brattelid and Finn Olav Levy.
505 8 $aDetermining allosteric modulator mechanism of action : integration of radioligand binding and functional assay data / Christopher J. Langmead -- Design and use of fluorescent ligands to study ligand-receptor interactions in single living cells / Stephen J. Briddon, Barrie Kellam, and Stephen J. Hill -- Examining site-specific GPCR phosphorylation / Adrian J. Butcher, Andrew B. Tobin, and Kok Choi Kong -- Ubiquitination of GPCRs / Adriana Caballero and Adriano Marchese -- [35S]GTPgS binding as an index of total G-protein and Ga-subtype-specific activation by GPCRs / Rajendra Mistry, Mark R. Dowling, and R. A. John Challiss -- Using calcium imaging as a readout of GPCR activation / Martin D. Bootman and H. Llewelyn Roderick -- Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors / Frank Gesellchen ... [et al.].
505 8 $aDetermining the activation of Rho as an index of receptor coupling to G12/13 proteins / Michio Nakaya ... [et al.] -- Use of translocating fluorescent biosensors for real-time monitoring of GPCR-mediated signaling events / Carl P. Nelson and R.A. John Challiss -- Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs / Ashley E. Brady ... [et al.] -- Study of GPCR-protein interactions by BRET / Martina Kocan and Kevin D.G. Pfleger -- Time resolved FRET strategy with fluorescent ligands to analyze receptor interactions in native tissues : application to GPCR oligomerization / Martin Cottet ... [et al.] -- Peptide affinity purification for the isolation and identification of GPCR-associated protein complexes / Pascal Maurice, Avais M. Daulat, and Ralf Jockers.
505 8 $aTandem affinity purification and identification of GPCR-associated protein complexes / Avais M. Daulat, Pascal Maurice, and Ralf Jockers -- Identification of GPCR localization in detergent resistant membranes / Ranju Kumari and Anna Francesconi -- Analysis of GPCR localization and trafficking / James N. Hislop and Mark von Zastrow -- Statistical methods in research / Domenico Spina.
655 2 $aLaboratory Manuals
650 12 $aSignal Transduction$xphysiology.
655 7 $aComputer network resources.$2local
700 1 $aWillars, Gary B.
700 1 $aChalliss, R. A. J.$q(R. A. John)
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$v746.
830 0 $aSpringer protocols.
988 $a20110614
906 $0OCLC