MARC Record from harvard_bibliographic_metadata

LEADER: 06730cam a2200565Ii 4500
001 013924317-8
005 20140314191604.0
006 m o d
008 131204s2014 nyua ob 001 0 eng d
020 $a9781627036702 (electronic bk.)
020 $a1627036709 (electronic bk.)
020 $z9781627036696 (print)
024 7 $a10.1007/978-1-62703-670-2$2doi
035 0 $aocn864681043
040 $aGW5XE$beng$erda$epn$cGW5XE$dYDXCP$dOHS$dFTU
050 4 $aQR414.5$b.H858 2014
072 7 $aMJCM$2bicssc
072 7 $aMED044000$2bisacsh
082 04 $a579.2/569$223
245 00 $aHuman retroviruses :$bmethods and protocols /$cedited by Elisa Vicenzi and Guido Poli, Division of Immunology, Transplant, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy.
264 1 $aNew York :$bHumana Press,$c[2014]
264 4 $c©2014
300 $a1 online resource (xviii, 345 pages) :$billustrations (some color).
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
490 1 $aMethods in Molecular Biology,$x1940-6029 ;$vVolume No. 1087
504 $aIncludes bibliographical references and index.
505 00 $gPart I.$tMapping the HIV Life Cycle --$tVirion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay /$rClaudia Cicala and James Arthos --$tCryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5[alpha] /$rGongpu Zhao and Peijun Zhang --$tThe Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating /$rYang Yang, Jeremy Luban, and Felipe Diaz-Griffero --$tThe Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells /$rAmy E. Hulme and Thomas J. Hope --$tImaging HIV-1 Nuclear Pre-integration Complexes /$rAnna Cereseto and Mauro Giacca --$tHIV-1 Reverse Transcription /$rAndrea Cimarelli and Jean-Luc Darlix --$tRNase H: Specificity, Mechanisms of Action, and Antiviral Target /$rKarin Moelling, Felix Broecker, and John E. Kerrigan --$tHIV-1 Chromatin, Transcription, and the Regulatory Protein Tat /$rLaurence Colin, Eric Verdin, and Carine Van Lint --$tHIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export /$rAlan Cochrane --$tHIV-1 Accessory Proteins: Nef /$rAnke Heigele, Daniel Sauter, Jan Münch, and Frank Kirchhoff --$tHIV-1 Accessory Proteins: VpR /$rRichard Y. Zhao and Michael I. Bukrinsky --$tHIV-1 Accessory Proteins: Vpu and Vif /$rAmy Andrew and Klaus Strebel --$tSIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells /$rGregory Berger and Andrea Cimarelli --$tImaging of HIV Assembly and Release /$rBarbara Müller and Jacomine Krijnse-Locker.
505 80 $gPart II.$tIsolation, Co-Receptor Use, and Cell Tropism of HIV-1 --$tHIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells /$rStefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, and Gabriella Scarlatti --$tDetermination of HIV-1 Co-receptor Usage /$rMariangela Cavarelli and Gabriella Scarlatti --$tThe Macrophage and HIV: Basic Concepts and Methodologies /$rSuzanne Gartner --$tHIV Infection of Dendritic Cells /$rNajla Nasr, Andrew Harman, Stuart Turville, and Anthony L. Cunningham --$tHistocultures (Tissue Explants) in Human Retrovirology /$rAnush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, and Leonid Margolis.
505 80 $gPart III.$tIn Vivo Quantification of HIV-1 --$tSingle-Copy Quantification of HIV-1 in Clinical Samples /$rAnn Wiegand and Frank Maldarelli --$tQuantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells /$rChristine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël.
505 80 $gPart IV.$tBiotechnological Aspects of HIV-1 --$tHIV-1-Based Lentiviral Vectors /$rYing Poi Liu and Ben Berkhout --$tQuantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone /$rZachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang.
505 80 $gPart V.$tHTLVS --$tInvestigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques /$rGreta Forlani, Roberto S. Accolla, and Giovanna Tosi --$tProviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR /$rClaudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni --$tQuantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real-Time RT-PCR (qRT-PCR) /$rIlaria Cavallari, Francesca Rende, and Vincenzo Ciminale.
520 $a"Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore assay and function of accessory genes, largely involving the interface between retroviral and host factors, the extracellular role of Tat and Tax, resembling the function of cytokines, and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field."--Publisher's description.
588 $aDescription based on online resource; title from PDF title page (Springer Protocols, viewed January 30, 2014).
650 20 $aDiagnosis, Laboratory.
650 20 $aVirology.
650 20 $aImmunology.
650 0 $aRetroviruses$vLaboratory manuals.
650 0 $aHIV (Viruses)$vLaboratory manuals.
650 0 $aHTLV (Viruses)$vLaboratory manuals.
650 12 $aRetroviridae$vLaboratory Manuals.
650 12 $aHIV$vLaboratory Manuals.
650 12 $aDeltaretrovirus$vLaboratory Manuals.
650 14 $aBiomedicine.
700 1 $aVicenzi, Elisa,$eeditor of compilation.$4edc
700 1 $aPoli, Guido,$eeditor of compilation.$4edc
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$v1087.$x1064-3745
988 $a20140205
906 $0OCLC