MARC Record from harvard_bibliographic_metadata

LEADER: 04585cam a2200517Ki 4500
001 013925083-2
005 20140314191633.0
006 m o d
008 140106s2014 nyua o 001 0 eng d
016 7 $a016607209$2Uk
020 $a9781627037150 (electronic bk.)
020 $a1627037152 (electronic bk.)
020 $z9781627037143
024 7 $a10.1007/978-1-62703-715-0$2doi
035 0 $aocn867545931
040 $aGW5XE$beng$erda$epn$cGW5XE$dYDXCP$dRML$dUKMGB
050 4 $aQK495.G74
072 7 $aPST$2bicssc
072 7 $aSCI011000$2bisacsh
072 7 $aNAT026000$2bisacsh
082 04 $a633.1$223
245 00 $aCereal genomics :$bmethods and protocols /$cedited by Robert J. Henry, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia, Agnelo Furtado, Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia.
264 1 $aTotowa, NJ :$bHumana Press,$c2014.
300 $a1 online resource (x, 300 pages) :$billustrations (some color).
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
490 1 $aMethods in Molecular Biology, Methods and Protocols,$x1064-3745 ;$v1099
505 0 $aDNA Extraction from Vegetative Tissue for Next Generation Sequencing? -- DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) -- RNA Extraction from Cereal Vegetative Tissue -- RNA Extraction from Developing or Mature Wheat Seeds -- cDNA Library Preparation -- Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants -- The Polymerase Chain Reaction (PCR) - General Methods -- Mutation and Mutation Screening -- The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression -- Cloning of DNA Fragments: Ligation Reactions in Agarose Gel -- Rapid Cloning of Genes and Promoters for Functional Analyses -- Genome Walking -- Functional Analysis by Protein Biochemistry -- Genomic Southern Blot analysis -- Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs -- Protein Blotting-Protocol for Beginners -- Genetic Transformation of Wheat via Particle Bombardment -- Sorghum Genetic Transformation by Particle Bombardment -- Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery -- A Protocol for High-throughput Agrobacterium-mediated Barley Transformation -- Agrobacterium-mediated Transformation- Rice Transformation -- Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos -- A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops -- A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data.
500 $aIncludes index.
520 $aIn Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes. Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described. Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.
588 $aDescription based on online resource; title from PDF title page (SpringerLink, viewed November 18, 2013).
504 $aIncludes bibliographical references and index.
650 10 $aLife sciences.
650 0 $aGrain$xGenetics$vLaboratory manuals.
650 24 $aPlant Sciences.
650 24 $aPlant Genetics & Genomics.
650 24 $aTransgenics.
650 2 $aCereals$xgenetics.
650 2 $aCereals.
650 2 $aBreeding.
700 1 $aHenry, Robert J.,$eeditor of compilation.
700 1 $aFurtado, Agnelo,$eeditor of compilation.
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$v1099.$x1064-3745
988 $a20140206
906 $0OCLC