MARC Record from harvard_bibliographic_metadata

LEADER: 03191nam a2200481Ki 4500
001 014047615-6
005 20140620195651.0
006 m o d
008 140217s2014 nyua ob 001 0 eng d
020 $a9781461496328 (electronic bk.)
020 $a1461496322 (electronic bk.)
020 $z9781461496311
024 7 $a10.1007/978-1-4614-9632-8$2doi
035 0 $aocn870649789
040 $aGW5XE$beng$erda$epn$cGW5XE
050 4 $aQH585.5.E48
082 04 $a571.96481$222
245 00 $aElectroporation protocols :$bpreclinical and clinical gene medicine /$cedited by Shulin Li, Jeffry Cutrera, Richard Heller, Justin Teissie.
250 $aSecond edition.
264 1 $aNew York :$bHumana Press,$c[2014]
264 4 $c©2014
300 $a1 online resource (xiv, 349 pages) :$billustrations.
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
490 1 $aMethods in molecular biology,$x1064-3745 ;$v1121
504 $aIncludes bibliographical references and index.
520 $aElectroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture, and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues, and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.
588 $aDescription based on online resource; title from PDF title page (SpringerLink, viewed Feb. 17, 2014).
650 12 $aElectroporation$xmethods.
650 12 $aGenetic Therapy$xmethods.
650 0 $aElectroporation.
650 0 $aGene therapy.
650 0 $aMobile genetic elements.
650 0 $aTransfection.
650 7 $aElectroporation.$2fast
650 7 $aGene therapy.$2fast
650 7 $aMobile genetic elements.$2fast
650 7 $aTransfection.$2fast
700 1 $aLi, Shulin,$cProf.
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 1121.
988 $a20140512
906 $0OCLC