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008 070802s1996 caua ob 001 0 eng d
006 m o d
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020 $a9780123423351
020 $a012342335X
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020 $a0080527906$q(electronic bk.)
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037 $a81008:81008$bElsevier Science & Technology$nhttp://www.sciencedirect.com
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060 4 $a1996 F-196
060 4 $aQU 25$bH552b 1996
070 $aQP517.B49H47$b1996
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100 1 $aHermanson, Greg T.
245 10 $aBioconjugate techniques /$cGreg T. Hermanson.
260 $aSan Diego :$bAcademic Press,$c℗♭1996.
300 $a1 online resource (xxv, 785 pages) :$billustrations
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
504 $aIncludes bibliographical references (pages 673-725) and index.
505 0 $aFunctional targets -- The chemistry of reactive groups -- Zero-length cross-linkers -- Homobifunctional cross-linkers -- Heterobifunctional cross-linkers -- Trifunctional cross-linkers -- Cleavable reagent systems -- Tags and probes -- Preparation of hapten-carrier immunogen conjugates -- Antibody modification and conjugation -- Immunotoxin conjugation techniques -- Preparation of liposome conjugates and derivatives -- Avidin-biotin systems -- Preparation of colloidal-gold-labeled proteins -- Modification with synthetic polymers -- Enzyme modification and conjugation -- Nucleic acid and oligonucleotide modification and conjugation.
520 $aBioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules. Key Features * This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volume * Serves as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeutics * Provides useful, detailed, easy-to-follow, step-by-step protocols * Contains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all types * Efficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugates * Cites over more than references keyed to concepts covered in the book * Uses more than 600 figures to illustrate bioconjugate reagents, their reactions, and applications * Suggests sources for all key reagents.
506 $3Use copy$fRestrictions unspecified$2star$5MiAaHDL
533 $aElectronic reproduction.$b[Place of publication not identified] :$cHathiTrust Digital Library,$d2010.$5MiAaHDL
538 $aMaster and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.$uhttp://purl.oclc.org/DLF/benchrepro0212$5MiAaHDL
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588 0 $aPrint version record.
650 0 $aBioconjugates$xSynthesis.
650 2 $aBiochemistry$xmethods.
650 2 $aProteins$xchemistry.
650 2 $aPeptides$xchemistry.
650 2 $aNucleic Acids$xchemistry.
650 7 $aSCIENCE$xLife Sciences$xBiochemistry.$2bisacsh
650 17 $aBiochemie.$2gtt
650 17 $aWetenschappelijke technieken.$2gtt
653 0 $aBiochemistry
655 4 $aElectronic books.
776 08 $iPrint version:$aHermanson, Greg T.$tBioconjugate techniques.$dSan Diego : Academic Press, ℗♭1996$z012342335X$z9780123423351$w(DLC) 95016511$w(OCoLC)32512549
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