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Source | Internet Archive |
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020 $a0199634777$q(v. 1)
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035 $a(OCoLC)30810745$z(OCoLC)34546483$z(OCoLC)34688324
050 00 $aQH442.2$b.D59 1994
060 4 $aQH 442.2$bD629
070 0 $aQH442.2.D59$b1995
072 0 $aX300
082 00 $a574.87/3282$220
084 $a35.73$2bcl
084 $a42.20$2bcl
245 00 $aDNA cloning :$ba practical approach /$cedited by D.M. Glover and B.D. Hames.
250 $a2nd ed.
260 $aOxford ;$aNew York :$bIRL Press,$c℗♭1994-
300 $a4 volumes :$billustrations ;$c24 cm
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
490 1 $aPractical approach series ;$vv. <148, 149>
504 $aIncludes bibliographical references and indexes.
505 0 $av. 1. Core techniques -- v. 2. Expression systems.
520 $aIn recent years, heterologous gene expression in mammalian cells has become invaluable for studying many aspects of eukaryote cell and molecular biology. This book provides key background information and detailed practical guidance on using the major expression systems currently available. The experienced authors describe laboratory protocols that they know work, and give clear technical advice, hints and tips, troubleshooting comments, and example results, all designed to ensure practical success.
520 8 $aTopics covered include: high-efficiency gene transfer into mammalian cells; construction and characterization of vaccinia virus recombinants; use of vectors based on gene amplification; retroviral vectors; genetic manipulation of embryonic stem cells; production of transgenic rodents by microinjection of cloned DNA; genomic and expression analysis of transgenic animals; expression using a defective HSV-1 vector system; adenovirus vectors.
520 8 $aThis volume, Mammalian Systems, is one of four new Practical Approach volumes on DNA cloning which together provide a comprehensive practical manual of current recombinant DNA methodology. Mammalian Systems will prove invaluable to all researchers, from PhD students to experienced group leaders, who already express heterologous genes in mammalian systems or who plan to do so.
650 0 $aMolecular cloning$vLaboratory manuals.
650 0 $aRecombinant DNA$vLaboratory manuals.
650 12 $aCloning, Molecular.
650 22 $aDNA, Recombinant.
650 7 $a42.20 genetics (biology)$0(NL-LeOCL)077603087$2bcl
650 7 $a35.73 molecular genetics.$0(NL-LeOCL)087981637$2bcl
650 7 $aMolecular cloning.$2fast$0(OCoLC)fst01024762
650 7 $aRecombinant DNA.$2fast$0(OCoLC)fst01091466
650 7 $aADN recombine .$2fmesh
650 7 $aClonage mole culaire.$2fmesh
650 17 $aKloneren.$2gtt
650 17 $aDNA.$2gtt
650 17 $aWetenschappelijke technieken.$2gtt
650 7 $aAcidos nucleicos.$2larpcal
650 7 $aGenetica molecular e de microorganismos.$2larpcal
650 7 $aClonage mole culaire$xManuels de laboratoire.$2ram
650 7 $aADN recombine $xManuels de laboratoire.$2ram
653 0 $aDNA$aSynthesis
655 2 $aLaboratory Manual.
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
700 1 $aGlover, David M.
700 1 $aHames, B. D.
776 08 $iOnline version:$tDNA cloning.$b2nd ed.$dOxford ; New York : IRL Press, ℗♭1994-$w(OCoLC)623971318
776 08 $iOnline version:$tDNA cloning.$b2nd ed.$dOxford ; New York : IRL Press, ℗♭1994-$w(OCoLC)626587700
830 0 $aPractical approach series ;$vv. <148, 149>.
856 41 $3Table of contents$uhttp://catdir.loc.gov/catdir/enhancements/fy0605/94026740-t.html
856 42 $3Publisher description$uhttp://catdir.loc.gov/catdir/enhancements/fy0605/94026740-d.html
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948 $hNO HOLDINGS IN P4A - 291 OTHER HOLDINGS