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LEADER: 06360cam 2200781Ia 4500
001 ocn300493342
003 OCoLC
005 20200522231053.0
008 090130s2009 nyuaf ob 001 0 eng d
006 m o d
007 cr unu||||||||
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016 7 $a101495639$2DNLM
019 $a1035782811$a1058404284$a1069556416
020 $a9781597452014$q(electronic bk.)
020 $a1597452017$q(electronic bk.)
020 $z9781588298836$q(alk. paper)
020 $z1588298833$q(alk. paper)
024 7 $a10.1007/978-1-59745-201-4$2doi
035 $a(OCoLC)300493342$z(OCoLC)1035782811$z(OCoLC)1058404284$z(OCoLC)1069556416
050 4 $aRD546
060 4 $aW1$bME9616J v.481 2009
060 4 $aWI 25$bH529 2009
072 7 $aPSB$2bicssc
072 7 $aSCI007000$2bisacsh
082 04 $a617.55620592$222
245 00 $aHepatocyte transplantation :$bmethods and protocols /$cedited by Anil Dhawan, Robin D. Hughes.
260 $aNew York :$bHumana Press,$c©2009.
300 $a1 online resource (xvii, 231 pages, 9 unnumbered pages of plates) :$billustrations
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
347 $atext file$bPDF$2rda
490 1 $aMethods in molecular biology,$x1064-3745 ;$v481
490 1 $aSpringer protocols
504 $aIncludes bibliographical references and index.
505 0 $aHuman hepatocyte transplantation overview / J. Puppi and A. Dhawan -- Isolation of human hepatocytes / R.R. Mitry -- An optimised method for cryopreservation of human hepatocytes / C. Terry and R.D. Hughes -- Liver cell culture techniques / J.V. Castell and M.J. Gomez-Lechon -- In vitro assays for induction of drug metabolism / B.G. Lake [and others] -- Hepatocyte apoptosis / M. Najimi, F. Smets and E. Sokal -- Small animal models of hepatocyte transplantation / J. Seppen, E.E. Filali and R.O. Elferink -- Hepatocyte transplantation techniques: large animal models / A. Weber, M.T. Groyer-Picard and I. Dagher -- Cell transplant techniques: engraftment detection of cells / R.A. Fisher and V.R. Mas -- Hepatic preconditioning for transplanted cell engraftment and proliferation / Y.M. Wu and S. Gupta -- Ex vivo gene transfer into hepatocytes / X. Wang [and others] -- Sources of adult hepatic stem cells: haematopoietic / R. Jeffery, R. Poulsom and M.R. Alison -- Production of hepatocyte-like cells from human amnion / T. Miki [and others] -- Generation of hepatocytes from human embryonic stem cells / N. Safinia and S.L. Minger -- Isolation, in vitro cultivation and characterisation of foetal liver cells / Y. Wu, C.C. Shatapathy and S.L. Minger -- Human intrahepatic biliary epithelial cell lineages: studies in vitro / R. Joplin and S. Kachilele -- Liver cell labelling with MRI contrast agents / M. Modo, T.J. Meade and R.R. Mitry -- Microbiological monitoring of hepatocyte isolation in the GMP laboratory / S.C. Lehec.
588 0 $aPrint version record.
520 $aDue to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.
546 $aEnglish.
650 0 $aLiver cells$xTransplantation.
650 12 $aLiver Diseases$xsurgery.
650 22 $aLiver$xcytology.
650 22 $aCell Transplantation.
650 17 $aHépatocytes$xtransplantation$xCahiers de laboratoire.$2fmesh
650 27 $aSéparation cellulaire$xméthodes$xCahiers de laboratoire.$2fmesh
650 7 $aCellules hépatiques$xTransplantation.$2ram
655 4 $aElectronic books.
700 1 $aDhawan, Anil.
700 1 $aHughes, Robin D.
776 08 $iPrint version:$tHepatocyte transplantation.$dNew York : Humana Press, ©2009$z9781588298836$w(DLC) 2008939645$w(OCoLC)236549611
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 481.
830 0 $aSpringer protocols (Series)
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856 40 $3SpringerLink$uhttp://springerlink.com/content/t33066/?p=a46d38fbd2fa4ea3953825245dfff029&pi=20
856 40 $3SpringerLink$uhttps://doi.org/10.1007/978-1-59745-201-4
856 40 $3SpringerLink$uhttp://link.springer.com/book/10.1007/978-1-59745-201-4
856 40 $3SpringerLink$uhttp://link.springer.com/10.1007/978-1-59745-201-4
856 40 $3SpringerLink$uhttps://link.springer.com/openurl?genre=book&isbn=978-1-58829-883-6
856 40 $3SpringerLink$uhttps://link.springer.com/book/10.1007/978-1-59745-201-4
856 40 $3SpringerLink$uhttp://www.library.yorku.ca/e/resolver/id/2527904
856 40 $3SpringerLink$uhttps://link.springer.com/10.1007/978-1-59745-201-4
856 40 $uhttps://0-link-springer-com.pugwash.lib.warwick.ac.uk/book/10.1007/978-1-59745-201-4$zConnect to Springer Protocols e-book
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