| Record ID | ia:introductiontoim0000pola_x2a4 |
| Source | Internet Archive |
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LEADER: 03900cam 2200601 a 4500
001 ocm51194081
003 OCoLC
005 20200520051350.0
008 021206s2003 enka b 001 0 eng
010 $a 2006389571
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020 $a9781859962084
020 $a1859962084
035 $a(OCoLC)51194081
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060 00 $a2003 C-441
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080 $a578.6
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100 1 $aPolak, Julia M.
245 10 $aIntroduction to immunocytochemistry /$cJ.M. Polak, S. Van Noorden.
250 $a3rd ed.
260 $aOxford :$bBIOS Scientific Publishers,$c2003.
300 $axv, 176 pages :$billustrations ;$c24 cm
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
500 $aPrevious edition: 1997.
504 $aIncludes bibliographical references and index.
505 00 $tHistory and development --$tProduction of Antibodies --$tImmunization --$tTesting --$tRegion-specific antibodies --$tMonoclonal antibodies --$tSelection of antibodies by phage display --$tCharacteristics of a 'good' antibody --$tPreparation of Tissue for Immunocytochemistry --$tFixation --$tCross-linking fixatives --$tPrecipitant fixatives --$tCombination fixatives --$tFixed, paraffin-embedded tissue --$tFresh material--frozen sections and cell preparations --$tFrozen sections --$tWhole cell preparations --$tPre-fixed, non-embedded tissue --$tPre-fixed frozen sections --$tPre-fixed Vibratome sections --$tWhole-mounts --$tPermeabilization --$tFreeze-dried tissue --$tTissue storage --$tParaffin blocks and sections --$tFrozen blocks and sections --$tCell preparations --$tAdherence of sections and cell preparations to slides --$tAntigen retrieval in fixed tissues --$tWashing --$tProtease treatment --$tHeat-mediated antigen retrieval --$tColour plate section --$tVisualizing the End-product of Reaction --$tFluorescent labels --$tAdvantages --$tDisadvantages --$tUses of immunofluorescence --$tFluorescein --$tRhodamine --$tPhycoerythrin --$tAMCA --$tOther fluorophores --$tFluorescent counterstains --$tEnzyme labels --$tPeroxidase --$tAlkaline phosphatase --$tGlucose oxidase --$t[beta]-d-Galactosidase --$tGold labels --$tColloidal gold --$tNanogold --$tOther labels --$tBiotin --$tHaptens --$tRadioisotopes --$tNon-specific Staining due to Tissue Factors --$tCauses of non-specific binding --$tCharged sites --$tHydrophobic attraction --$tFc receptors.
520 $aTwo members of the Imperial College faculty of medicine describe the direct, indirect, three-layer, and avidin-biotin methods of immunocytochemistry for localizing tissue constituents with labeled antibodies, and offer advice on how to prepare samples and get the best results. An appendix lists reagents and protocols. The third edition adds new met.
650 0 $aImmunocytochemistry.
650 12 $aImmunohistochemistry.
650 7 $aImmunocytochemistry.$2fast$0(OCoLC)fst00967934
650 17 $aImmunocytochemie.$2gtt
650 17 $aWetenschappelijke technieken.$2gtt
700 1 $aVan Noorden, Susan.
856 41 $3Table of contents$uhttp://catdir.loc.gov/catdir/toc/fy0610/2006389571.html
938 $aBaker & Taylor$bBKTY$c75.00$d.00$i1859962084$n0004203847$sactive
938 $aBrodart$bBROD$n01109332$c$59.95
938 $aYBP Library Services$bYANK$n100332469
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994 $aZ0$bP4A
948 $hNO HOLDINGS IN P4A - 206 OTHER HOLDINGS