MARC record from Internet Archive

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001 ocn227382816
003 OCoLC
005 20200511012146.0
008 080509s2008 njua ob 001 0 eng d
006 m o d
007 cr mn|||||||||
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020 $a9781603271691$q(Springer e-ISBN)
020 $a1603271694$q(Springer e-ISBN)
020 $z9781588297716$q(alk. paper)
020 $z1588297713$q(alk. paper)
024 7 $a10.1007/978-1-60327-169-1$2doi
035 $a(OCoLC)227382816$z(OCoLC)771208886$z(OCoLC)964891904$z(OCoLC)967621887$z(OCoLC)990607439$z(OCoLC)1005762518$z(OCoLC)1017916909$z(OCoLC)1043059897$z(OCoLC)1058382516$z(OCoLC)1071631822$z(OCoLC)1081278652$z(OCoLC)1087339184
050 4 $aQH588.S83$bM47 2008
060 4 $aW1$bME9616J v.449 2008
060 4 $aQU 325$bM578 2008
072 7 $aPSF$2bicssc
072 7 $aSCI049000$2bisacsh
082 04 $a616/.02774$222
245 00 $aMesenchymal stem cells :$bmethods and protocols /$cedited by Darwin J. Prockop, Donald G. Phinney, Bruce A. Bunnell.
260 $aTotowa, N.J. :$bHumana Press,$c℗♭2008.
300 $a1 online resource (xvi, 192 pages) :$billustrations (some color)
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
347 $atext file$bPDF$2rda
490 1 $aMethods in molecular biology ;$v449
504 $aIncludes bibliographical references and index.
505 0 $apt. I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe [and others] ; Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger ; A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C.W. Zannettino ; Adipose-derived stem cells / John K. Fraser [and others] ; Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois [and others] -- pt. II. Manipulation of human multipotential stromal cells ; Colony forming unit assays for MSCs / Radhika Pochampally ; Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe ; Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe ; Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi ; Assays of MSCs with microarrays / Joni Yo stalo, Radhika Pochampally, and Darwin J. Prockop ; Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- pt. III. Isolation and characterization of murine multipotential stromal cells from bone marrow ; Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney.
588 0 $aPrint version record.
520 $aFor over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In Mesenchymal Stem Cells: Methods and Protocols, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Recently, these vital cells have shown therapeutic benefits in the treatment of myocardial infarction, stroke, lung diseases, spinal cord injury and other neurological disorders, thus promising a boundless future in their study. Written to the specifications of the highly successful Methods in Molecular Biology series format, each chapter contains step-by-step laboratory protocols, a list of the necessary materials, and a Notes section with tips on troubleshooting and avoiding known pitfalls. Cutting edge and easy to use, Mesenchymal Stem Cells: Methods and Protocols is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.
546 $aEnglish.
650 0 $aStem cells$vLaboratory manuals.
650 12 $aMesenchymal Stem Cells.
650 22 $aCytological Techniques.
650 7 $aStem cells.$2fast$0(OCoLC)fst01132975
650 7 $aCytology.$2hilcc
650 7 $aBiology.$2hilcc
650 7 $aHealth & Biological Sciences.$2hilcc
653 00 $astamcellen
653 00 $astem cells
653 00 $abeenmergcellen
653 00 $abone marrow cells
653 00 $aceldifferentiatie
653 00 $acell differentiation
653 10 $aCellular Biology
653 10 $aCelbiologie
655 4 $aElectronic books.
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
700 1 $aProckop, Darwin J.
700 1 $aPhinney, Donald G.
700 1 $aBunnell, Bruce A.
773 0 $tSpringer Protocols
776 08 $iPrint version:$tMesenchymal stem cells.$dTotowa, NJ : Humana Press, ℗♭2008$z9781588297716$w(DLC) 2007938053$w(OCoLC)216935882
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 449.
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856 40 $3SpringerLink$uhttp://link.springer.com/10.1007/978-1-60327-169-1
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