MARC record from Internet Archive

LEADER: 05644cam a22004097a 4500
001 2007926773
003 DLC
005 20080506081838.0
008 070412s2008 njua b 001 0 eng d
010 $a 2007926773
020 $a9781588293787 (acid-free paper)
020 $a1588293785 (acid-free paper)
035 $a(OCoLC)ocn209342501
040 $aUMM$cUMM$dBAKER$dCOU$dPIT$dDLC
042 $alccopycat
050 00 $aQH434$b.M495 2008
245 00 $aMicrobial gene essentiality :$bprotocols and bioinformatics /$cedited by Andrei L. Osterman and Svetlana Y. Gerdes.
260 $aTotowa, N.J. :$bHumana Press,$cc2008.
300 $axiv, 486 p. :$bill. ;$c27 cm.
490 1 $aMethods in molecular biology ;$v416
504 $aIncludes bibliographical references and index.
505 0 $aOverview of whole-genome essentiality analysis / Karen Joy Shaw -- Pt. I. Experimental protocols. IA. Populational genome-wide essentiality screens. Transposon-based strategies for the identification of essential bacterial genes / William S. Reznikoff and Kelly M. Winterberg -- Identification and analysis of essential genes in Haemophilus influenzae / Sandy M.S. Wong and Brian J. Akerley -- Transposon site hybridization in Mycobacterium tuberculosis / Jeffrey P. Murry ... [et al.] -- Essential genes in the infection model of Pseudomonas aeruginosa PCR-based signature-tagged mutagenesis / François Sanschagrin, Irena Kukavica-Ibrulj, and Roger C. Levesque -- Whole-genome detection of conditionally essential and dispensable genes in Escherichia coli via genetic footprinting / Michael D. Scholle and Svetlana Y. Gerdes -- Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis / Taeok Bae ... [et al.] -- Multipurpose transposon insertion libraries for large-scale analysis of gene function in yeast / Anuj Kumar --
505 8 $aIB. Systematic collections of knockout mutants. How to make a defined near-saturation mutant library. Case 1: Pseudomonas aeruginosa PAO1 / Michael A. Jacobs -- Comparing insertion libraries in two Pseudomonas aeruginosa strains to assess gene essentiality / Nicole T. Liberati ... [et al.] -- The construction of systematic in-frame, single-gene knockout mutant collection in Escherichia coli K-12 / Tomoya Baba and Hirotada Mori -- The applications of systematic in-frame, single-gene knockout mutant collection of Escherichia coli K-12 / Tomoya Baba ... [et al.] -- A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12 / Takeyoshi Miki, Yoshihiro Yamamoto, and Hideo Matsuda -- High-throughput creation of a whole-genome collection of yeast knockout strains / Angela M. Chu and Ronald W. Davis -- Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray / Pamela B. Meluh ... [et al.] --
505 8 $aIC. Genome minimization. Scarless engineering of the Escherichia coli genome / Tamás Fehér ... [et al.] -- Minimization of the Escherichia coli genome using the Tn5-targeted Cre/loxP excision system / Byung Jo Yu and Sun Chang Kim -- Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome / Jun-ichi Kato and Masayuki Hashimoto -- ID. Conditional knockouts. Identification of essential genes in Staphylococcus aureus by construction and screening of conditional mutant library / Dezhong Yin and Yinduo Ji -- Techniques for the isolation and use of conditionally expressed antisense RNA to achieve essential gene knockdowns in Staphylococcus aureus / Allyn Forsyth and Liangsu Wang -- Introduction of conditional lethal amber mutations in Escherichia coli / Christopher D. Herring -- Pt. II. Bioinformatics. IIA. Statistics. Statistical methods for building random transposon mutagenesis libraries / Oliver Will -- Statistical evaluation of genetic footprinting data / Gábor Balázsi -- Modeling competitive outgrowth of mutant populations : why do essentiality screens yield divergent results? / Alexander I. Grenov and Svetlana Y. Gerdes --
505 8 $aStatistical analysis of fitness data determined by TAG hybridization on microarrays / Brian D. Peyser, Rafael Irizarry, and Forrest A. Spencer -- IIB. Data integration and modeling. Profiling of Escherichia coli chromosome database / Yukiko Yamazaki, Hironori Niki, and Jun-ichi Kato -- Gene essentiality analysis based on DEG, a database of essential genes / Chun-Ting Zhang and Ren Zhang -- Detection of essential genes in Streptococcus pneumoniae using bioinformatics and allelic replacement mutagenesis / Jae-Hoon Song and Kwan Soo Ko -- Design and application of genome-scale reconstructed metabolic models / Isabel Rocha, Jochen Förster, and Jens Nielsen -- Predicting gene essentiality using genome-scale in silico models / Andrew R. Joyce and Bernhard Ø. Palsson -- Comparative approach to analysis of gene essentiality / Andrei L. Osterman and Svetlana Y. Gerdes.
650 0 $aMicrobial genetics.
650 0 $aComputational biology.
650 12 $aGenes, Essential$xgenetics$vLaboratory Manuals.
650 12 $aGenetics, Microbial$vLaboratory Manuals.
650 22 $aGenome, Bacterial$xgenetics$vLaboratory Manuals.
650 22 $aComputational Biology$vLaboratory Manuals.
650 22 $aGenomics$xmethods$vLaboratory Manuals.
650 22 $aGenetic Techniques$vLaboratory Manuals.
650 22 $aMicrobiological Techniques$vLaboratory Manuals.
700 1 $aOsterman, Andrei L.
700 1 $aGerdes, Svetlana Y.
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 416.