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LEADER: 06288cam 2200949 a 4500
001 ocm40331082
003 OCoLC
005 20200918210840.0
008 981026s1999 ne a b 001 0 eng
010 $a 98043502
040 $aDNLM/DLC$beng$cDLC$dOHX$dAGL$dNLM$dBAKER$dBTCTA$dYDXCP$dOCLCG$dZWZ$dBDX$dOCLCF$dOCLCO$dOCLCQ$dNAM$dOCLCQ$dUWO$dWURST$dOCLCA
016 7 $a100883072$2DNLM
020 $a0817659730$q(Boston ;$qhardcover ;$qalk. paper)
020 $a9780817659738$q(Boston ;$qhardcover ;$qalk. paper)
020 $a3764359730$q(Basel ;$qhardcover ;$qalk. paper)
020 $a9783764359737$q(Basel ;$qhardcover ;$qalk. paper)
020 $a0817660194$q(Boston ;$qwire-o binding ;$qalk. paper)
020 $a9780817660192$q(Boston ;$qwire-o binding ;$qalk. paper)
020 $a3764360194$q(Basel ;$qwire-o binding ;$qalk. paper)
020 $a9783764360191$q(Basel ;$qwire-o binding ;$qalk. paper)
035 $a(OCoLC)40331082
050 00 $aQH585.5.M52$bM53 1999
060 00 $a1999 E-841
060 10 $aQH 585$bM6263 1999
070 0 $aQH585.5.M52$bM53 1999
072 7 $aQH$2lcco
072 0 $aX300
082 00 $a571.6/07/2$221
245 00 $aMicroinjection /$cedited by Juan Carlos Lacal, Rosario Perona, James Feramisco.
260 $aBasel ;$aBoston :$bBirkhäuser Verlag,$c©1999.
300 $a248 pages :$billustrations ;$c25 cm.
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
490 1 $aMethods and tools in biosciences and medicine
504 $aIncludes bibliographical references and index.
505 0 $a1. Needle Microinjection: A Brief History / J. Feramisco, R. Perona and J.C. Lacal -- 2. Microinjection of Macromolecules into Mammalian Cells in Culture / R. Perona, F. Dolfi and J. Feramisco / [and others] -- 3. The Use of Plasmid Microinjection to Study Specific Cell Cycle Phase Transitions / E.S. Knudsen -- 4. Combining Microinjection with Microanalytical Methods to Investigate Regulation of Metabolic Pathways / J. Manchester and J.C. Lawrence, Jr. -- 5. Import of Stably Folded Proteins into Peroxisomes / P.A. Walton -- 6. Study of the Function of Microtubule Proteins / R. Armas-Portela and J. Avila -- 7. Cytoskeleton Regulation by Rho Proteins / R. Kozma, S. Ahmed and L. Lim -- 8. Cytoskeletal Regulation by Rho-p21 Associated Kinases: Analysis of ROK and PAK Function by Plasmid Microinjection / T. Leung, E. Manser and L. Lim -- 9. Use of Microinjection to Study Apoptosis and its Prevention / F. Kohlhuber, H. Hermeking and D. Eick -- 10. Role of the InsPase and Casuperscript 2+] Entry / R.S. Mathias and H.E. Ives -- 11. Microinjection of Xenopus laevis Oocytes: A Model System / J.C. Lacal -- 12. Xenopus laevis Oocytes as a Model for Studying the Activation of Intracellular Kinases / A. Carnero and J.C. Lacal -- 13. Signal Transduction in Xenopus laevis Oocytes by Ras Oncoproteins and Lipid Metabolites / A. Carnero and J.C. Lacal -- 14. The Multiple Roles of Mos During Meiosis / M.S. Murakami and G.F. Vande Woude -- 15. Use of the Xenopus Oocyte System to Study RNA Transport / L.A. Allison -- 16. Functional Expression of G Protein-Coupled Receptors in Xenopus laevis Oocytes / P. de la Pena and F. Barros -- 17. Expression of Glucose Transporters in Xenopus laevis Oocytes: Applications for the Study of Membrane Transporter Function and Regulation / M.J. Seatter and G.W. Gould -- 18. DNA Injection into Xenopus laevis Embryos as a Tool to Study Spatial Gene Activity / M. Kuhl, M. Walter and J. Clement / [and others].
505 8 $a19. Transformation of Nematodes by Microinjection / S. Hashmi, G. Hashmi and R. Gaugler -- Guide to Solutions -- Guide to Protocols -- Troubleshooting Guide.
520 1 $a"The technique of needle microinjection has become a prominent experimental approach in biological research."--BOOK JACKET. "This book presents information on the technique's principles, the required equipment, preparation of recipient cells and injected material and the analysis of the results. It provides detailed protocols for a wide range of important applications in different cellular systems from the fields of cell cycle regulation, signal transduction, study of transcriptional regulations, cytoskeletal functions, secretion and intracellular transport. Because of its outstanding relevance in cell biology, a large part of the book is dedicated to the Xenopus laevis oocyte system."--BOOK JACKET.
650 0 $aMicroinjections.
650 0 $aCytology$xTechnique.
650 12 $aCytological Techniques.
650 12 $aMicroinjections$xmethods.
650 22 $aGenetic Engineering$xmethods.
650 22 $aTransformation, Genetic$xgenetics.
650 7 $aCytology$xTechnique.$2fast$0(OCoLC)fst00886306
650 7 $aMicroinjections.$2fast$0(OCoLC)fst01019877
650 7 $aMésothérapie.$2ram
650 7 $aCytologie$xTechnique.$2ram
653 00 $ainjectie
653 00 $ainjection
653 00 $amicromanipulatie
653 00 $amicromanipulation
653 00 $acytologie
653 00 $acytology
653 00 $agenetische modificatie
653 00 $agenetic engineering
653 00 $agenetische transformatie
653 00 $agenetic transformation
653 10 $aGenetic Engineering
653 20 $aBiological Techniques
653 10 $aGenetische modificatie
653 20 $aBiologische technieken
700 1 $aLacal, Juan Carlos.
700 1 $aPerona, Rosario,$d1956-
700 1 $aFeramisco, James.
830 0 $aMethods and tools in biosciences and medicine.
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938 $aBaker & Taylor$bBKTY$c55.01$d55.01$i0817660194$n0003234508$sactive
938 $aBaker & Taylor$bBKTY$c194.00$d194.00$i3764359730$n0003234507$sactive
938 $aBrodart$bBROD$n54828082$c$30.95
938 $aBaker and Taylor$bBTCP$n98043502
938 $aOtto Harrassowitz$bHARR$nhar005071655
938 $aYBP Library Services$bYANK$n7967038
029 1 $aAU@$b000014266060
029 1 $aNLGGC$b186675089
029 1 $aNLM$b100883072
029 1 $aOHX$bhar005071655
029 1 $aYDXCP$b1544745
029 1 $aYDXCP$b1596931
994 $aZ0$bP4A
948 $hNO HOLDINGS IN P4A - 115 OTHER HOLDINGS