MARC record from Internet Archive

LEADER: 06320cam 2200841 a 4500
001 ocm38307871
003 OCoLC
005 20200508203716.0
008 980203s1998 njua b 001 0 eng d
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042 $alccopycat
050 04 $aQP535.N1$bN557 1998
060 00 $aW1$bME9616J v.100 1998
060 10 $aQV 25$bN731 1998
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082 00 $a572/.54$221
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245 00 $aNitric oxide protocols /$cedited by Michael A. Titheradge.
260 $aTotowa, N.J. :$bHumana Press,$c℗♭1998.
300 $axi, 324 pages :$billustrations ;$c24 cm.
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
490 1 $aMethods in molecular biology ;$vv. 100
504 $aIncludes bibliographical references and index.
505 0 $aEnzymology of nitric oxide synthases / Benjamin Hemmens and Bernd Mayer -- Purification of the inducible nitric oxide synthase ; Purification of the constitutive nitric oxide synthase / Edward P. Garvey -- Cloning and expression of human inducible nitric oxide synthase / Mark L. Johnson, Richard A. Shapiro, Timothy R. Billiar -- Cloning and expression of human eNOS and nNos using the baculovirus-insect cell system / Ian G. Charles, Neale Foxwell, Ann Chubb -- Assay of NOS activity by the measurement of conversion of oxyhemoglobin to methemoglobin by NO / Mark Salter and Richard G. Knowles -- Measurement of NOS activity by conversion of radiolabeled arginine to citrulline using ion-exchange separation / Richard G. Knowles and Mark Salter -- Radiochemical measurement of NOS activity by conversion of [℗£þ́C]L-arginine to citrulline using HPLC separation / James M. Cunningham and Richard C. Rayne -- The enzymatic measurement of nitrate and nitrite / Michael A. Titheradge -- Determination of NOS activity using cyclic-GMP formation / Suzanne G. Laychock -- Determination of NO with a Clark-type electrode / Kurt Schmidt and Bernd Mayer -- The measurement of NO in biological systems using chemiluminescence / Evangelos D. Michelakis and Stephen L. Archer -- Measurement of NO using electron paramagnetic resonance / S. Tsuyoshi Ohnishi -- Measurement of eNOS and iNOS mRNA expression using reverse transcription polymerase chain reaction / Norbert Reiling, Artur J. Ulmer, Sunna Hauschildt -- Measurement of NOS mRNA by Northern blotting and the ribonuclease-protection assay / Paloma Marti n-Sanz and Lisardo Bosca -- Detection of NOS isoforms by Western-blot analysis / Fiona S. Smith and Michael A. Titheradge --Immunohistochemical localization of NOS isoforms / Victoria Cattell and Karen Mosley -- A practical protocol for the demonstration of NOS using in-situ hybridization / G. Michael Taylor and H. Terence Cook -- Use of NO donors in biological systems / Jayne M. Tullett and Daryl D. Rees -- Making and working with peroxynitrite / Roger White [and others] -- The use of NO gas in biological systems / Neil Hogg and B. Kalyanaraman -- A microtiter-plate assay of human NOS isoforms / John Dawson and Richard G. Knowles -- Use of arginine analogs as inhibitors of nitric oxide synthase in rat-aortic rings / Rachel J. Russell -- Measurement of biopterin and the use of inhibitors of biopterin biosynthesis / Kazuyuki Hatakeyama -- A sensitive assay for the enzymatic activity of GTP cyclohydrolase I / Kazuyuki Hatakeyama and Toshie Yoneyama -- Simultaneous measurement of mitochondrial function and NO / Ignacio Lizasoain and Mari a A. Moro -- Release of NO from donor compounds : a mathematical model for calculation of NO concentrations in the presence of oxygen / Kurt Schmidt [and others] -- The determination of nitrotyrosine residues in proteins / Andrew J. Gow [and others] -- Measurement of DNA damage using the comet assay / Steven Thomas [and others] -- Methods for the study of NO-induced apoptosis in cultured cells / Anne C. Loweth and Noel G. Morgan.
520 $aDesigned for both novice and experienced researchers, this volume presents time-tested techniques to cover all aspects of nitric oxide synthase and metabolism.
650 0 $aNitric oxide$xMetabolism.
650 0 $aNitric oxide$xSynthesis.
650 12 $aNitric Oxide$xisolation & purification.
650 12 $aNitric Oxide$xmetabolism.
650 7 $aNitric oxide$xMetabolism.$2fast$0(OCoLC)fst01037988
650 17 $aStikstofmonoxide.$2gtt
650 17 $aChemische analyses.$2gtt
650 7 $aMethode$2gnd
650 7 $aMolekularbiologie$2gnd
650 7 $aStickstoffoxide$2gnd
655 2 $aLaboratory Manual.
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
655 7 $aLaboratory manuals.$2lcgft
700 1 $aTitheradge, Michael A.
776 08 $iOnline version:$tNitric oxide protocols.$dTotowa, N.J. : Humana Press, ℗♭1998$w(OCoLC)606955190
776 08 $iOnline version:$tNitric oxide protocols.$dTotowa, N.J. : Humana Press, ℗♭1998$w(OCoLC)607881172
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 100.
856 4 $uhttps://link.springer.com/book/10.1385/1592597491
856 4 $uhttp://www.springerprotocols.com/BookToc/doi/10.1385/1592597491
938 $aBaker & Taylor$bBKTY$c104.50$d104.50$i0896035379$n0003191518$sactive
938 $aBaker and Taylor$bBTCP$n98132223
938 $aYBP Library Services$bYANK$n1503364
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948 $hNO HOLDINGS IN P4A - 171 OTHER HOLDINGS