MARC record from Internet Archive

LEADER: 08996cam 2201057 a 4500
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003 OCoLC
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008 960731s1997 njua b 001 0 eng
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020 $z0896034436$q(hardcover ;$qalk. paper)
020 $a0896033430$q(combbound ;$qalk. paper)
020 $a9780896033436$q(combbound ;$qalk. paper)
020 $a0896034836
020 $a9780896034839
020 $a9781592595532
020 $a1592595537
035 $a(OCoLC)35192222$z(OCoLC)39445779$z(OCoLC)187420921
050 00 $aQH442.2$b.P37 1997
060 00 $aW1$bME9616J v.67 1997
060 10 $aQH 442.2$bP348 1997
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245 00 $aPCR cloning protocols :$bfrom molecular cloning to genetic engineering /$cedited by Bruce A. White.
260 $aTotowa, N.J. :$bHumana Press,$c℗♭1997.
300 $axiv, 490 pages :$billustrations ;$c23 cm.
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
490 1 $aMethods in molecular biology ;$vv. 67
504 $aIncludes bibliographical references and index.
505 0 $aPCR : basic principles and routine practice / Lori A. Kolmodin, J. Fenton Williams -- XL PCR amplification of long targets from genomic DNA / Suzanne Cheng, Lori A. Kolmodin -- Amplification of DNA sequences up to 5 kb from small amounts of genomic DNA using tub DNA polymerase / Helen B. Forrester, Ian R. Radford -- One-step optimization using touchdown and stepdown PCR / Kenneth H. Roux, Karl H. Hecker -- GC-rich template amplification by inverse PCR : DNA polymerase and solvent effects / Alain Moreau, Colette Duez, Jean Dusart -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments / Jyrki T. Aatsinki -- Using T4 DNA polymerase to generate clonable PCR products / Kai Wang -- Rapid (ligase-free) subcloning of PCR products / Alan R. Shuldiner, Keith Tanner -- Cloning PCR products utilizing the T/A overhang and a kit / Melissa Lail-Trecker -- Cloning unmodified PCR products using engineered Xcml restriction sites in a portable cassette / Alessandro Testori, Paul Sollitti -- A T-linker strategy for modification and directional cloning of PCR products / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine -- Recovery of DNA amplification products from silver-stained polyacrylamide gels : applications in nucleic acid fingerprinting and genetic mapping / Gustavo Caetano-Anolle s, Robert N. Trigiano -- Recombination and site-directed mutagenesis using recombination PCR / Douglas H. Jones, Stanley C. Winistorfer -- In vitro recombination and mutagenesis of DNA : SOEing together tailor-made genes / Robert M. Horton -- In-frame cloning of synthetic genes using PCR inserts / James C. Pierce -- Creation of chimeric junctions, deletions, and insertions by PCR / Genevie ve Pont-Kingdon -- Mutagenesis and gene fusion by megaprimer PCR / Sailen Barik -- Rapid and efficient one-tube PCR-based mutagenesis method / Veronique Picard, Susan Clark Bock -- Thermostable ligase-mediated incorporation of mutagenic oligonucleotides during PCR amplification / Scott F. Michael -- Linker scanning mutagenesis by three-step PCR / Judith T. Schanke -- Sequence inversion by flip-PCR / Judith T. Schanke -- PCR site-directed mutagenesis using pyrococcus sp GB-D polymerase coupled to a rapid screening procedure : application to a [beta]-glucanase gene / Jaume Pons, Antoni Planas, Miquel Juncosa, Enrique Querol -- Using the SELEX combinatorial chemistry process to find high affinity nucleic acid ligands to target molecules / Craig Tuerk -- Rapid amplification of cDNA ends / David Bertioli -- Amplification of gene-regulating regions with single-sided specificity / Mei-Zhong Luo, Rino Cella -- An end-trimming method and its application to amplify adjacent cDNA and genomic DNA fragments by PCR / Hiroyuki Iwahana, Mitsuo Itakura -- Anchoring a defined sequence to the 5' ends of mRNAs : the bolt to clone rare full-length mRNAs / Jean Baptiste Edwards, Olivier Valdenaire, Jacques Mallet -- Rapid directional walk within DNA clones by step-out PCR / Umadevi V. Wesley, Cedric S. Wesley -- Inverse PCR : an efficient approach to cloning cDNA ends / Sheng-He Huang -- Rapid amplification of gene ends (RAGE) from gene libraries by anchored PCR / Sheng-He Huang, Ambrose Y. Jong -- Isolation of coding sequences from yeast artificial chromosome (Yac) : clones by exon amplification / Fernando Gibson, Steve D. Brown -- cDNA libraries from a low amount of cells / Philippe Ravassard, Christine Icard-Liepkalns, Jacques Mallet, Jean Baptiste Edwards -- Rapid and nonradioactive screening of recombinant libraries by PCR / Michael W. King -- Use of PCR for cDNA library screening / Toru Takumi -- Generation and PCR screening of bacteriophage [lambda] sublibraries enriched for rare clones (the "sublibrary method") / Michael Lardelli -- Normalization of cDNA sequence representation by molecular selection / Thierry G. Coche -- Subtractive cDNA cloning using magnetic beads and PCR / Thierry G. Coche -- Generation of a PCR-renewable source of subtractive cDNA / W. Michael Kuehl, James Battey -- The use of PCR for differential screening of cDNA libraries / Mark G. Thomas, Sarah A. Hesse, Yvonne J. Foss, Farzin Farzaneh -- Identification and cloning of differentially expressed genes by DDRT-PCR / Mikkel Rohde, Rene Hummel, Niels Pallisgaard, Tino Podstufka, Heidi Riedel, Henrik Leffers, Michael Strauss -- Cloning gene family members using PCR with degenerate oligonucleotide primers / Gregory M. Preston -- Amplification using degenerate primers with multiple inosines to isolate genes with minimal sequence similarity / Simona Bartl -- Designing PCR primers to amplify specific members or subgroups of multigene families / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine -- Screening gene family-enriched cDNA sublibraries with an unamplified cDNA probe : focusing on moderately to abundantly expressed clones / Meimei Hu, Bruce A. White.
520 $aThis book provides extensive protocols on the use of PCR for molecular cloning, including the cloning of known sequences, cloning of unknown sequences and cloning of gene family members.
650 0 $aMolecular cloning$vLaboratory manuals.
650 0 $aPolymerase chain reaction$vLaboratory manuals.
650 12 $aCloning, Molecular.
650 22 $aPolymerase Chain Reaction.
650 6 $aRe action en chai ne de la polyme rase$xTechnique.
650 6 $aClonage mole culaire$xTechnique.
650 6 $aADN polyme rases$xTechnique.
650 6 $aAmplification ge nique$xTechnique.
650 6 $aMutagene se dirige e$xTechnique.
650 7 $aMolecular cloning.$2fast$0(OCoLC)fst01024762
650 7 $aPolymerase chain reaction.$2fast$0(OCoLC)fst01070553
650 17 $aPolymerase kettingreactie.$2gtt
650 17 $aGenetische manipulatie.$2gtt
650 17 $aWetenschappelijke technieken.$2gtt
650 17 $aKloneren.$2gtt
650 7 $aGenklonierung$2gnd
650 7 $aMethode$2gnd
650 7 $aPolymerase-Kettenreaktion$2gnd
650 7 $aAufsatzsammlung$2gnd
653 0 $aMolecular cloning
653 0 $aPolymerase chain reaction
655 2 $aLaboratory Manual.
655 4 $aAufsatzsammlung.
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
655 7 $aLaboratory manuals.$2lcgft
700 1 $aWhite, Bruce Alan.
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 67.
856 4 $uhttp://www.springerprotocols.com/BookToc/doi/10.1385/0896034836
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