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LEADER: 06807cam 2200961 a 4500
001 ocm31412122
003 OCoLC
005 20201023201410.0
008 941019s1995 caua b 001 0 eng
010 $a 94042506
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015 $aGB9568302$2bnb
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016 7 $a9513140$2DNLM
019 $a1167078660$a1171659116
020 $a0123721822$q(case ;$qacid-free paper)
020 $a9780123721822$q(case ;$qacid-free paper)
020 $a0123721830$q(paper ;$qacid-free paper)
020 $a9780123721839$q(paper ;$qacid-free paper)
035 $a(OCoLC)31412122$z(OCoLC)1167078660$z(OCoLC)1171659116
037 $cNo price
050 00 $aQP606.D46$bP367 1995
060 00 $a1995 H-882
060 10 $aQP 606.D46$bP3482 1995
070 0 $aQP606.D46P367$b1995
072 0 $aX300
080 $a577.213.3$bP346
080 $a577.196.3
082 00 $a574.87/3282$220
084 $a35.75$2bcl
245 00 $aPCR strategies /$cedited by Michael A. Innis, David H. Gelfand, and John J. Sninsky.
246 3 $aPolymerase chain reaction strategies
260 $aSan Diego :$bAcademic Press,$c©1995.
300 $axv, 373 pages :$billustrations ;$c24 cm
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
504 $aIncludes bibliographical references and index.
520 $aPCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a set of up-to-date strategies and protocols for using PCR in all relevant research areas.
505 0 $a1. The Use of Cosolvents to Enhance Amplification by the Polymerase Chain Reaction / P. A. Landre, D. H. Gelfand and R. M. Watson -- 2. DNA Polymerase Fidelity: Misinsertions and Mismatched Extensions / Myron F. Goodman -- 3. Extraction of Nucleic Acids: Sample Preparation from Paraffin-Embedded Tissues / Hiroko Shimizu and Jane C. Burns -- 4. Thermostable DNA Polymerases / Richard D. Abramson -- 5. Amplification of RNA: High-Temperature Reverse Transcription and DNA Amplification with Thermus thermophilus DNA Polymerase / Thomas W. Myers and Christopher L. Sigua -- 6. Nucleic Acid Hybridization and Unconventional Bases / James G. Wetmur and John J. Sninsky -- 7. Practical Considerations for the Design of Quantitative PCR Assays / Robert Diaco -- 8. Carrier Detection of Cystic Fibrosis Mutations Using PCR-Amplified DNA and a Mismatch-Binding Protein, MutS / Alla Lishanski and Jasper Rine -- 9. Single-Stranded Conformational Polymorphisms / Anne L. Bailey -- 10. Analysis of PCR Products by Covalent Reverse Dot Blot Hybridization / Farid F. Chehab, Jeff Wall and Shi-ping Cai -- 11. High-Performance Liquid Chromatography Analysis of PCR Products / John M. Wages, Xumei Zhao and Elena D. Katz -- 12. Heteroduplex Mobility Assays for Phylogenetic Analysis / Eric L. Delwart, Eugene G. Shpaer and James I. Mullins -- 13. PCR Amplification of VNTRs / S. Scharf -- 14. Site-Specific Mutagenesis Using the Polymerase Chain Reaction / Jonathan Silver, Teresa Limjoco and Stephen Feinstone -- 15. Exact Quantification of DNA-RNA Copy Numbers by PCR-TGGE / Jie Kang, Peter Schafer, Joachim E. Kuhn, Andreas Immelmann and Karsten Henco -- 16. The in Situ PCR: Amplication and Detection of DNA in a Cellular Context / Ernest Retzel, Katherine A. Staskus, Janet E. Embretson and Ashley T. Haase -- 17. Y Chromosome-Specific PCR: Maternal Blood / Diana W. Bianchi -- 18. Genomic Subtraction / Don Straus -- 19. DNA Amplification-Restricted Transcription Translation (DARTT): Analysis of in Vitro and in Situ Protein Functions and Intermolecular Assembly / Erich R. Mackow -- 20. DNA and RNA Fingerprinting Using Arbitrarily Primed PCR / John Welsh, David Ralph and Michael McClelland -- 21. PCR-Based Screening of Yeast Artificial Chromosome Libraries / Eric D. Green -- 22. Oligonucleotide Ligands That Discriminate between Theophylline and Caffeine / Rob Jenison, Stanley Gill, Jr. and Barry Polisky -- 23. Generation of Single-Chain Antibody Fragments by PCR / Jeffrey R. Stinson, Vaughan Wittman and Hing C. Wong -- 24. Longer PCR Amplifications / Suzanne Cheng -- 25. Direct Analysis of Specific Bonds from Arbitrarily Primed PCR Reactions / D. A. Carter, A. Burt and J. W. Taylor -- 26. Detection of Leber's Hereditary Optic Neuropathy by Nonradioactive Ligase Chain Reaction / John A. Zebala and Francis Barany -- 27. Detection of Listeria monocytogenes by PCR-Coupled Ligase Chain Reaction / Martin Wiedmann, Francis Barany and Carl A. Batt.
650 0 $aPolymerase chain reaction$xMethodology.
650 7 $aPolymerase chain reaction$xMethodology.$2fast$0(OCoLC)fst01070556
650 7 $aRéaction de polymérisation en chaîne$xMéthodes.$2fmesh
650 7 $aDNA-directed DNA polymerase.$2fmesh
650 7 $aDNA-directed RNA polymerases.$2fmesh
650 7 $aAcides nucléiques$xanalyse.$2fmesh
650 17 $aPolymerase kettingreactie.$2gtt
650 17 $aWetenschappelijke technieken.$2gtt
650 7 $aRéaction en chaîne de la polymérase.$2ram
650 12 $aPolymerase Chain Reaction.
653 $aCadena de reacción de la polimerasa.
653 $aADN polimerasa.
653 $aARN polimerasa.
653 0 $aDNA
653 00 $apolymerase-kettingreactie
653 00 $apolymerase chain reaction
653 00 $agenetische modificatie
653 00 $agenetic engineering
653 00 $amethodologie
653 00 $amethodology
653 10 $aNucleic Acids
653 20 $aPlant Breeding and Genetics (General)
653 20 $aPlantenveredeling en -genetica (algemeen)
655 2 $aLaboratory Manual.
700 1 $aInnis, Michael A.
700 1 $aGelfand, David H.
700 1 $aSninsky, John J.
856 41 $3Table of contents$uhttp://catdir.loc.gov/catdir/toc/els032/94042506.html
856 42 $3Publisher description$uhttp://catdir.loc.gov/catdir/description/els032/94042506.html
938 $aBaker & Taylor$bBKTY$c76.95$d76.95$i0123721830$n0002667519$sactive
938 $aBaker & Taylor$bBKTY$c155.00$d155.00$i0123721822$n0002584639$sactive
938 $aBrodart$bBROD$n48899984$c$126.95
938 $aBaker and Taylor$bBTCP$n94042506
938 $aPuvill Libros$bPUVL$n0123721830$c$68.97
938 $aYBP Library Services$bYANK$n57479
029 1 $aAU@$b000011259803
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029 1 $aNLM$b9513140
029 1 $aNZ1$b4658095
029 1 $aYDXCP$b57478
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994 $aZ0$bP4A
948 $hNO HOLDINGS IN P4A - 318 OTHER HOLDINGS