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245 00 $aProtocols for neural cell culture /$cedited by Sergey Fedoroff and Arleen Richardson.
250 $a3rd ed.
260 $aTotowa, N.J. :$bHumana Press,$c©2001.
300 $a1 online resource (xxii, 362 pages) :$billustrations
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
490 1 $aSpringer Protocols Handbooks
504 $aIncludes bibliographical references and index.
505 0 $aIn vitro assays for axonal growth and targeting / Valérie Castellani and Jürgen Bolz -- Interface organotypic hippocampal slice cultures / Dominique Muller [and others] -- Slice cultures for study of microglia / Carol A. Colton, Meggan Czapiga, and Toby N. Behar -- Microexplant cultures of the cerebellum / Bernard Rogister and Gustave Moonen -- Construction and use of compartmented cultures for studies of cell biology of neurons / Robert B. Campenot and Grace Martin -- Chick spinal somatic motoneurons in culture / Bernhard H.J. Juurlink -- Primary cultures of sympathetic ganglia / Mary I. Johnson -- Primary cell cultures for the study of myelination / Mary I. Johnson, Richard P. Bunge, and Patrick M. Wood -- Preparation of astrocyte, oligodendrocyte, and microglia cultures for primary rat cerebral cultures / Ruth Cole and Jean de Vellis -- Culture of glial cells from human brain biopsies / V. Wee Yong and Jack P. Antel.
505 0 $aCultures of astroglia and microglia from primary cultures of mouse neopallium / Sergey Fedoroff and Arleen Richardson -- Cultures of oligodendroblasts from primary cultures of rat neopallium / Bernhard H.J. Juurlink, Shawn K. Thorburne, and Richard M. Devon -- Olfactory ensheathing cell cultures / Ronald Doucette -- Cultures of stem cells of the central nervous system / Angela Gritti, Rossella Galli, and Angelo Luigi Vescovi -- Aggregating neural cell cultures / Paul Honegger and Florianne Monnet-Tschudi -- Neural cell lines / Kaoru Murayama [and others] -- Virus vectors for gene therapy of the nervous system / Sonia N. Yeung and Frank Tufaro -- Preparation of substrata for in vitro culture of neurons / Paul C. Letourneau -- Serum-fee media for neural cell cultures: adult and embryonic / Paul J. Price and Gregory J. Brewer.
505 0 $aDevelopment of multiple-well plate biological assays / John w. Harbell -- Neural cell counting: cell number determination in microplate cultures / Marston Manthorpe -- Assays for neuronal cell migration / Toby N. Behar -- Colony culture: plating efficiency assay and cloning / Sergey Fedoroff and Arleen Richardson -- Hybridoma cultures for production of antibodies: purification and specificity of antibodies / J. Denry Sato -- Elimination of cell types from mixed neural cell cultures / Richard M. Devon -- Quantification of cells in culture / Arleen Richardson and Sergey Fedoroff -- Tips for tissue culture / Arleen Richardson and Sergey Fedoroff.
520 $aIn Protocols for Neural Cell Culture, Third Ed., Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as essential procedures for hippocampal and microglial slice cultures and transfection of neurons in culture with adenovirus. It includes key techniques for the preparation of substrata, the use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. For scientists not trained in neuroanatomy, but faced with dissecting the brain and spinal cord, most chapters in the 3rd edition provide fully detailed dissection procedures. Protocols for Neural Cell Culture, Third Ed. is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture. Its unique wealth of practical detail on a wide range of tissue culture systems having many applications ensure that this new edition will remain an essential resource for all investigators using cell culture methodology in studying the brain and its disorders.
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650 0 $aNerve tissue$xCultures and culture media$vLaboratory manuals.
650 0 $aNeurons$xGrowth$vLaboratory manuals.
650 12 $aNerve Tissue$xcytology.
650 22 $aTissue Culture$xmethods
650 22 $aNeurons$xcytology.
650 22 $aCulture Techniques$xmethods.
650 6 $aTissu nerveux$xCultures et milieux de culture.
650 6 $aNeurones$xCroissance.
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655 2 $aLaboratory Manual.
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655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
700 1 $aFedoroff, Sergey.
700 1 $aRichardson, Arleen.
776 08 $iPrint version:$tProtocols for neural cell culture.$b3rd ed.$dTotowa, N.J. : Humana Press, ©2001$z0896039021$w(DLC) 00059763$w(OCoLC)44678489
830 0 $aSpringer protocols (Series)
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