MARC record from Internet Archive

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001 ocn436089328
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005 20200526195924.0
008 090908s2009 nyua ob 001 0 eng d
006 m o d
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020 $a9781607612322$q(Springer e-ISBN)
020 $a1607612321$q(Springer e-ISBN)
020 $z9781607612315
020 $z1607612313
024 7 $a10.1007/978-1-60761-232-2$2doi
035 $a(OCoLC)436089328$z(OCoLC)645894931$z(OCoLC)681504943$z(OCoLC)771207557$z(OCoLC)908332314$z(OCoLC)964916060$z(OCoLC)967620590$z(OCoLC)972012588$z(OCoLC)990540728$z(OCoLC)1005834931$z(OCoLC)1027488018$z(OCoLC)1058396775$z(OCoLC)1069615637$z(OCoLC)1081244135$z(OCoLC)1087319885
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050 4 $aQH430
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070 $aQH430$b.R48 2009
072 7 $aMFN$2bicssc
072 7 $aMED107000$2bisacsh
082 04 $a572.8$222
245 00 $aReverse chemical genetics :$bmethods and protocols /$cedited by Hisashi Koga.
260 $aNew York, NY :$bHumana Press,$c©2009.
300 $a1 online resource (xi, 272 pages) :$billustrations (some color)
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
347 $atext file$bPDF$2rda
490 1 $aSpringer protocols
490 1 $aMethods in molecular biology,$x1940-6029 ;$v577
504 $aIncludes bibliographical references and index.
505 0 $aORFeome cloning / Osamu Ohara -- Systematic cloning of an ORFeome using the gateway system / Akihisa Matsuyama and Minoru Yoshida -- High-throughput construction of ORF clones for production of the recombinant proteins / Hisashi Yamakawa -- Computational overview of GPCR gene universe to support reverse chemical genomics study / Makiko Suwa and Yukiteru Ono -- High-performance gene expression module analysis tool and its application to chemical toxicity data / Wataru Fujibuchi [and others] -- Human protein reference database and human proteinpedia as discovery tools for systems biology / T.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh Pandey -- High-throughput production of the recombinant proteins expressed in Escherichia coli utilizing cDNA resources / Kiyo Shimada and Hisashi Koga -- An insect cell-free system for recombinant protein expresion using cDNA resources / Takashi Syuzuki [and others] -- Recombinant protein production by a kaiko-baculovirus system / Hidekazu Nagaya -- Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins / Kei Yamaguchi [and others] -- Application of 2D-DIGE in cancer proteomics toward personalized medicine / Tadashi Kondo and Setsuo Hirohashi -- Fully automated two-dimensional electrophoresis system for high-throughput protein analysis / Atsunori Hiratsuka and Kenji Yokoyama -- Large-scale whole mount in situ hybridization of mouse embryos / Hirohito Shimizu, Kenta Uchibe, and Hiroshi Asahara -- Identification of the specific binding proteins of bioactive small coumpound using affinity resins / Akito Tanaka -- Protein microarrays : effective tools for the study of inflammatory diseases / Xiaobo Yu [and others] -- Preparation of highly sensitive protein array using reactive polymer / Toshifumi Shiroya [and others] -- High-throughput SPR biosensor / Motoki Kyo [and others] -- Drug discovery through functional screening in the Drosophila heart / Takeshi Akasaka and Karen Ocorr -- Permeable cell assay : a method for high-throughput measurement of cellular ATP synthetic activity / Kiyotaka Y. Hara -- Novel methodology for immobilization of biomolecules on the surface of a photoresponsible polymer containing azobenzene moiety / Osamu Watanabe.
588 0 $aPrint version record.
520 $aNow a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study.
506 $3Use copy$fRestrictions unspecified$2star$5MiAaHDL
533 $aElectronic reproduction.$b[Place of publication not identified] :$cHathiTrust Digital Library,$d2010.$5MiAaHDL
538 $aMaster and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.$uhttp://purl.oclc.org/DLF/benchrepro0212$5MiAaHDL
583 1 $adigitized$c2010$hHathiTrust Digital Library$lcommitted to preserve$2pda$5MiAaHDL
546 $aEnglish.
650 0 $aBiochemical genetics.
650 2 $aMethods.
650 2 $aMolecular Biology.
650 2 $aLaboratory Manuals
650 2 $aCombinatorial Chemistry Techniques.
650 2 $aGenetic Techniques.
650 2 $aInvestigative Techniques
650 2 $aPublication Formats
650 2 $aBiochemistry.
650 2 $aTechnology, Pharmaceutical.
650 2 $aGenetics.
650 2 $aBiological Science Disciplines.
650 2 $aAnalytical, Diagnostic and Therapeutic Techniques and Equipment
650 2 $aBiology.
650 2 $aPublication Characteristics
650 2 $aChemistry.
650 2 $aNatural Science Disciplines.
650 2 $aDisciplines and Occupations
650 7 $aBiochemical genetics.$2fast$0(OCoLC)fst00831949
650 7 $aBiochemie$2gnd
650 7 $aGenetik$2gnd
650 7 $aLabortechnik$2gnd
650 7 $aMethode$2gnd
653 00 $agenetica
653 00 $agenetics
653 00 $amoleculaire genetica
653 00 $amolecular genetics
653 00 $adna cloning
653 00 $arecombinant eiwitten
653 00 $arecombinant proteins
653 00 $aeiwitexpressieanalyse
653 00 $aproteomics
653 10 $aMolecular Genetics
653 10 $aMoleculaire genetica
655 2 $aLaboratory Manual.
655 4 $aElectronic books.
700 1 $aKoga, Hisashi.
776 08 $iPrint version:$tReverse Chemical Genetics.$dHumana Press, 2009$z9781607612315$w(DLC) 2009933592$w(OCoLC)401153915
830 0 $aSpringer protocols (Series)
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 577.$x1064-3745
856 40 $3Google$uhttp://books.google.com/books?id=lWvwAAAAMAAJ
856 40 $3HathiTrust Digital Library, Limited view (search only)$uhttp://catalog.hathitrust.org/api/volumes/oclc/401153915.html
856 40 $3SpringerLink$uhttps://doi.org/10.1007/978-1-60761-232-2
856 40 $3SpringerLink$uhttps://link.springer.com/book/10.1007/978-1-60761-232-2
856 40 $3SpringerLink$uhttps://link.springer.com/book/10.1007/978-1-60761-231-5
856 40 $uhttp://www.library.yorku.ca/e/resolver/id/2479824
856 40 $uhttp://www.library.yorku.ca/e/resolver/id/2479825
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