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001 ocn166230185
003 OCoLC
005 20210515040307.0
008 070821s2001 caua ob 001 0 eng d
006 m o d
007 cr cnu---unuuu
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020 $a9780080496917$q(electronic bk.)
020 $a0080496911$q(electronic bk.)
020 $a0121822427$q(electronic bk.)
020 $a9780121822422$q(electronic bk.)
035 $a(OCoLC)166230185$z(OCoLC)170957969$z(OCoLC)808616159
037 $a9780080496917$bIngram Content Group
050 4 $aQP601$b.M49eb vol. 341
060 4 $aW1$bME9615K v.341 2001
060 4 $aQU 136$bR48249 2001
072 7 $aSCI$x007000$2bisacsh
082 04 $a572/.7$222
245 00 $aRibonucleases.$nVolume 341,$pPart A /$cedited by Allen W. Nicholson.
260 $aSan Diego :$bAcademic Press,$c©2001.
300 $a1 online resource (xxxvi, 734 pages) :$billustrations
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
347 $adata file
490 1 $aMethods in enzymology ;$vv. 341, pt. A
504 $aIncludes bibliographical references and indexes.
588 0 $aPrint version record.
520 $aThis first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and assays for secreted ribonucleases; viral ribonucleases, artificial and engineered ribonucleases, and ribozymes. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
505 0 $aFront Cover; Ribonucleases; Copyright Page; Table of Contents; Contributors to Volume 341; Preface; Volume in Series; Section I: Ribonuclease Classification and Review; Chapter 1. A Natural Classification of Ribonucleases; Chapter 2. The Ribonuclease T1 Family; Chapter 3. Ribonuclease T2; Chapter 4. The Ribonuclease P Family; Section II: Ribonuclease Assays; Chapter 5. Fast, Facile, Hypersensitive Assays for Ribonucleolytic Activity; Chapter 6. Activity Staining for Detection of Ribonucleases Using Dried Agarose Film Overlay Method after Isoelectric Focusing.
505 8 $aChapter 7. Gel Renaturation Assay for RibonucleasesChapter 8. Analysis of Ribonucleases Following Gel Electrophoresis; Chapter 9. Ribonuclease Assays Utilizing Toluidine Blue Indicator Plates, Methylene Blue, or Fluorescence Correlation Spectroscopy; Chapter 10. Ribonuclease Activities of Trypanosome RNA Editing Complex Directed to Cleave Specifically at a Chosen Site; Chapter 11. Ribonuclease YI*, RNA Structure Studies, and Variable Single-Strand Specificities of RNases; Section III: Secreted Ribonucleases.
505 8 $aChapter 12. Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding StudiesChapter 13. Purification of Engineered Human Pancreatic Ribonuclease; Chapter 14. Degradation of Double-Stranded RNA by Mammalian Pancreatic-Type Ribonucleases; Chapter 15. Seminal Ribonuclease: Preparation of Natural and Recombinant Enzyme, Quaternary Isoforms, Isoenzymes, Monomeric Forms; Assay for Selective Cytotoxicity of the Enzyme; Chapter 16. Angiogenin; Chapter 17. Eosinophil-Derived Neurotoxin; Chapter 18. Eosinophil Cationic Protein; Chapter 19. Deciphering the Mechanism of RNase T1.
505 8 $aChapter 20. Mitogillin and Related Fungal RibotoxinsChapter 21. RNase U2 and a-Sarcin: A Study of Relationships; Chapter 22. Secretory Acid Ribonucleases from Tomato, Lycopersicon esculentum Mill.; Chapter 23. Leczyme; Section IV: Ribonucleases H; Chapter 24. Prokaryotic Type 2 RNases H; Chapter 25. RNase H1 of Saccharomyces cerevisiae: Methods and Nomenclature; Chapter 26. Ribonucleases H of the Budding Yeast, Saccharomyces cerevisiae; Chapter 27. Human RNases H; Chapter 28. Assays for Retroviral RNase H; Section V: Synthetic Ribonucleases.
505 8 $aChapter 29. Sequence-Selective Artificial RibonucleasesChapter 30. RNA Cleavage by 1,4-Diazabicyclo[2.2.2]- octane-Imidazole Conjugates; Chapter 31. Preparation and Use of ZFY-6 Zinc Finger Ribonuclease; Section VI: Ribonucleolytic Nucleic Acids; Chapter 32. RNA Cleavage by the 10-23 DNA Enzyme; Chapter 33. Leadzyme; Chapter 34. Hammerhead Ribozyme Structure and Function in Plant RNA Replication; Chapter 35. Kinetic Analysis of Bimolecular Hepatitis delta Ribozyme; Chapter 36. Catalytic and Structural Assays for the Hairpin Ribozyme; Chapter 37. Intracellular Applications of Ribozymes.
650 0 $aRibonucleases.
650 12 $aRibonucleases.
650 6 $aRibonucléases.
650 7 $aSCIENCE$xLife Sciences$xBiochemistry.$2bisacsh
650 7 $aRibonucleases.$2fast$0(OCoLC)fst01097316
655 4 $aElectronic books.
700 1 $aNicholson, Allen W.
776 08 $iPrint version:$tRibonucleases. Pt. A.$dSan Diego : Academic Press, ©2001$z0121822427$z9780121822422$w(OCoLC)48193898
830 0 $aMethods in enzymology ;$vvolume 341, pt. A.
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