MARC record from Internet Archive

LEADER: 05221cam 2200865 a 4500
001 ocm40166639
003 OCoLC
005 20200928030332.0
008 990317s1998 caua b 001 0 eng
010 $a 98084574
040 $aDLC$beng$cDLC$dVYC$dIXA$dLVB$dNLM$dNLGGC$dBTCTA$dYDXCP$dNLE$dDEBBG$dZWZ$dTULIB$dOCLCF$dINNDH$dOCLCO$dOCLCQ$dIOG$dDHA$dOCLCA$dOCLCQ$dIAD$dOCLCO$dBGU$dUKMGB$dOCLCQ$dOCLCO$dOCLCQ
016 7 $a100884206$2DNLM
016 7 $a010216256$2Uk
019 $a778942124
020 $a0122496957
020 $a9780122496950
035 $a(OCoLC)40166639$z(OCoLC)778942124
050 00 $aQP623$b.F37 1998
060 00 $aQU 25$bF245r 1998
080 $a577.15 N8
082 00 $a572.8/8$221
084 $a35.75$2bcl
084 $aKE 91$2blsrissc
084 $aWC 4460$2rvk
084 $aWC 4150$2rvk
084 $aWD 5355$2rvk
086 1 $aQU 58 F245r 1998
100 1 $aFarrell, Robert E.,$cJr.
245 10 $aRNA methodologies :$ba laboratory guide for isolation and characterization /$cRobert E. Farrell, Jr.
250 $a2nd ed.
260 $aSan Diego :$bAcademic Press,$c©1998.
300 $axx, 533 pages :$billustrations ;$c23 cm
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
504 $aIncludes bibliographical references and index.
520 1 $a"This laboratory guide is a collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. The more noteworthy additions to this edition include a variety of RNA-based PCR techniques, such as differential display PCR and transcript quantification by competitive (mimic) PCR." "The focus of the book is on how these methodologies can be used in the study of transcriptional and posttranscriptional regulation of eukaryotic genes. The liberal incorporation of flow charts, tables, and graphs facilitates learning and assists in the planning phases of projects. Investigators are limited only by their own ingenuity."--Jacket.
505 00 $tPreface --$gCh. 1$tRNA and the Cellular Biochemistry Revisited --$gCh. 2$tTranscription and the Organization of Eukaryotic Gene Sequences --$gCh. 3$tMessenger RNA --$gCh. 4$tResilient Ribonucleases --$gCh. 5$tRNA Isolation Strategies --$gCh. 6$tIsolation of Polyadenylated RNA --$gCh. 7$tQuality Control for RNA Preparations --$gCh. 8$tDot Blot Analysis --$gCh. 9$tElectrophoresis of RNA --$gCh. 10$tPhotodocumentation and Image Analysis --$gCh. 11$tThe Northern Analysis --$gCh. 12$tNucleic Acid Probe Technology --$gCh. 13$tPractical Nucleic Acid Hybridization --$gCh. 14$tPrinciples of Detection --$gCh. 15$tRt Pcr --$gCh. 16$tMessenger RNA Differential Display --$gCh. 17$tQuantitative Polymerase Chain Reaction Techniques --$gCh. 18$tQuantification of Specific Messenger RNAs by Nuclease Protection --$gCh. 19$tAnalysis of Nuclear RNA --$gCh. 20$tAn RNA Paradigm --$tEpilogue: A Few Pearls of Wisdom --$gApp.$tA Phenol Preparation --$gApp. B$tDisposal of Ethidium Bromide and SYBR Green Solutions --$gApp. C$tDNase 1 Removal of DNA from an RNA Sample --$tGlossary --$tIndex.
650 0 $aRNA$xAnalysis$vLaboratory manuals.
650 12 $aRNA$xisolation & purification.$0https://id.nlm.nih.gov/mesh/D012313Q000302
650 22 $aMolecular Biology$xmethods.$0https://id.nlm.nih.gov/mesh/D008967Q000379
650 22 $aRNA$xphysiology.$0https://id.nlm.nih.gov/mesh/D012313Q000502
650 6 $aARN$xAnalyse$xManuels de laboratoire.
650 7 $aRNA$xAnalysis.$2fast$0(OCoLC)fst01086245
610 27 $aInstitut für Ressourcenschonende und Nachhaltige Systeme$2gnd
650 7 $aIsolierung$gChemie$2gnd
650 7 $aIsolierung$gMikrobiologie$2gnd
650 17 $aRNA.$2gtt
650 17 $aIsolering (proces)$2gtt
650 17 $aKarakterisering (algemeen)$2gtt
650 17 $aWetenschappelijke technieken.$2gtt
650 7 $aExpression génique.$2ram
650 7 $aAmplification génique.$2ram
650 7 $aCellules eucaryotes.$2ram
650 7 $aARN.$2ram
650 07 $aRNS.$2swd
655 2 $aLaboratory Manual.$0https://id.nlm.nih.gov/mesh/D020484
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
856 41 $3Table of contents$uhttp://catdir.loc.gov/catdir/toc/els032/98084574.html
856 41 $3Table of contents$uhttp://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=008313637&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
856 41 $3Table of contents$uhttp://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008313637&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
856 42 $3Publisher description$uhttp://catdir.loc.gov/catdir/description/els033/98084574.html
938 $aBaker and Taylor$bBTCP$n98084574
938 $aYBP Library Services$bYANK$n100129725
029 1 $aAU@$b000014292142
029 1 $aDEBBG$bBV012266375
029 1 $aHEBIS$b06257891X
029 1 $aHEBIS$b084259191
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029 1 $aNLM$b100884206
029 1 $aNZ1$b2539484
029 1 $aUKMGB$b010216256
029 1 $aYDXCP$b100129725
994 $aZ0$bP4A
948 $hNO HOLDINGS IN P4A - 204 OTHER HOLDINGS