MARC record from Internet Archive

LEADER: 05261cam 2200853 a 4500
001 ocm42352909
003 OCoLC
005 20160206120510.0
008 990910s2000 cauaf b 001 0 eng
007 ta
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084 $aciliater flimmerdyr protister encellede dyr fors©ıksorganismer$2utk
245 00 $aTetrahymena thermophila /$cedited by David J. Asai, James D. Forney.
260 $aSan Diego, CA :$bAcademic Press,$c℗♭2000.
300 $axviii, 580 pages, [5] pages of plates :$billustrations (some color) ;$c25 cm.
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
490 1 $aMethods in cell biology,$x0091-679X ;$vv. 62
500 $a"Prepared under the auspices of the American Society for Cell Biology."
504 $aIncludes bibliographical references and index.
505 00 $gPart I. Overview.$g1.$tlaboratory and evolutionary history of Tetrahymena thermophila --$g2.$tCell biology of Tetrahymena thermophila$g3.$tTetrahymena genetics: two nuclei are better than one --$gPart II. Methods.$g4.$tTetrahymena as a laboratory organism: useful strains, cell culture, and cell line maintenance --$g5.$tLong-term storage --$g6.$tGenetic crosses: setting up crosses, testing progeny, and isolating phenotypic assortants --$g7.$tMethods for genetic analysis --$g8.$tIsolation of micronuclear and macronuclear DNA --$g9.$tGenetically sorting a collection of Tetrahymena mutants --$g10.$tGenetically mapping new mutants and cloned genes --$g11.$tSelection of motility mutants --$g12.$tNuclear and cytoskeletal fluorescence microscopy techniques --$g13.$tNuclear and cortical histology for brightfield microscopy --$g14.$tFixation of Tetrahymena cells for electron microscopy --$g15.$tImmunoelectron microscopy of Tetrahymena --$gPart III. Cell biology. 16.$tRegulated protein secretion in Tetrahymena thermophila --$g17.$tElectrophysiology of Tetrahymena --$g18.$tIsolation and characterization of in vivo modified histones and an activity gel assay for identification of histone acetyltransferases --$g19.$tTetrahymena telomerase activity, purification, and reconstitution --$g20.$tStudying the telomerase RNA in Tetrahymena --$g21.$tIsolation and characterization of 22S outer arm dyein from Tetrahymena cilia --$g22.$tPreparation of cytoskeletal fractions from Tetrahymena thermophila --$g23.$tImmunoprecipitation procedures --$g24.$tTetrahymena calcium-binding proteins, TCBP-25 and TCBP-23 --$gPart IV.$tManipulating genes.$g25.$tMicroinjection of Tetrahymena thermophila --$g26.$tTransient and stable DNA transformation of Tetrahymena thermophila by electroporation --$g27.$tBiolistic transformation of macro- and micronuclei --$g28.$tKnockout heterokaryons enable facile mutagenic analysis of essential genes in Tetrahymena --$g29.$tCreation and use of antisense ribosomes in Tetrahymena thermophila --$g30.$tProtein tagging in Tetrahymena.
650 0 $aTetrahymena.
650 12 $aTetrahymena thermophila.
650 22 $aCell Culture Techniques.
650 6 $aTetrahymena.
650 7 $aTetrahymena.$2fast$0(OCoLC)fst01148410
650 17 $aProtozoa.$2gtt
650 07 $aTetrahymena thermophila.$2swd
650 07 $aCytologie.$2swd
650 07 $aAufsatzsammlung.$2swd
700 1 $aAsai, David J.
700 1 $aForney, James D.,$d1957-
710 2 $aAmerican Society for Cell Biology.
830 0 $aMethods in cell biology ;$vv. 62.
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