MARC Record from marc_columbia

LEADER: 02060mam a2200325 a 4500
001 1444785
005 20220602035713.0
008 930910t19941994nyua b 001 0 eng
010 $a 93034771
020 $a0471589667 (acid-free paper)
035 $a(OCoLC)ocm28929072
035 $9AHW4437CU
035 $a1444785
040 $aDLC$cDLC$dTMV$dNOH
050 00 $aQH585.2$b.F74 1994
082 00 $a591/.0724$220
100 1 $aFreshney, R. Ian.$0http://id.loc.gov/authorities/names/n83021058
245 10 $aCulture of animal cells :$ba manual of basic technique /$cR. Ian Freshney.
250 $a3rd ed.
260 $aNew York :$bWiley-Liss,$c[1994], ©1994.
300 $axxiv, 486 pages :$billustrations ;$c29 cm
336 $atext$2rdacontent
337 $aunmediated$2rdamedia
338 $avolume$2rdacarrier
504 $aIncludes bibliographical references (p. 437-469) and index.
505 2 $a1. Introduction -- 2. Biology of the Cultured Cell -- 3. Design and Layout of the Laboratory -- 4. Equipping the Laboratory -- 5. Aseptic Technique -- 6. Laboratory Safety and Biohazards -- 7. The Culture Environment: Substrate, Gas Phase, Medium and Temperature -- 8. Preparation and Sterilization -- 9. Disaggregation of the Tissue and Primary Culture -- 10. Maintenance of the Culture: Cell Lines -- 11. Cloning and Selection of Specific Cell Types -- 12. Physical Methods of Cell Separation -- 13. Characterization -- 14. Induction of Differentiation -- 15. The Transformed Phenotype -- 16. Contamination -- 17. Instability, Variation, and Preservation -- 18. Quantitation and Experimental Design -- 19. Measurement of Viability and Cytotoxicity -- 20. Culture of Specific Cell Types -- 21. Culture of Tumor Tissue -- 22. Three-Dimensional Culture Systems -- 23. Specialized Techniques.
650 0 $aTissue culture.$0http://id.loc.gov/authorities/subjects/sh85135588
650 0 $aCell culture.$0http://id.loc.gov/authorities/subjects/sh85021642
650 2 $aCells, Cultured.$0https://id.nlm.nih.gov/mesh/D002478
852 00 $bsci$hQH585.2$i.F74 1994