MARC Record from marc_columbia

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001 4242788
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020 $a9781592592159$q(electronic bk.)
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020 $z9780896033603$q(alk. paper)
020 $a1280830220
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024 7 $a10.1385/1592592155.$2doi
035 $a(OCoLC)232311803$z(OCoLC)45842676$z(OCoLC)82463684$z(OCoLC)232301985$z(OCoLC)320970055$z(OCoLC)533107546$z(OCoLC)655488644$z(OCoLC)771203035$z(OCoLC)961662641$z(OCoLC)962706081$z(OCoLC)964889424$z(OCoLC)967626897$z(OCoLC)970749953$z(OCoLC)984635960$z(OCoLC)990468185$z(OCoLC)994782933$z(OCoLC)1005762959$z(OCoLC)1007391292$z(OCoLC)1035703248$z(OCoLC)1053031602$z(OCoLC)1055842709$z(OCoLC)1071908643$z(OCoLC)1078826727$z(OCoLC)1078833852$z(OCoLC)1078847526$z(OCoLC)1081209319$z(OCoLC)1097293475$z(OCoLC)1135441264$z(OCoLC)1164898700$z(OCoLC)1166215537$z(OCoLC)1175935634$z(OCoLC)1229763536$z(OCoLC)1264832303
050 4 $aQH442.3$b.G46 2000eb
060 4 $aW1$bME9616J v.133 1999
060 4 $aQH 442.3$bG326 1999
072 7 $aSCI$x049000$2bisacsh
072 7 $aMFN.$2bicssc
082 04 $a572.877$221
049 $aZCUA
245 00 $aGene targeting protocols /$cedited by Eric B. Kmiec.
260 $aTotowa, N.J. :$bHumana Press,$c©2000.
300 $a1 online resource (xv, 244 pages) :$billustrations (some color)
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
490 1 $aMethods in molecular biology ;$vv. 133
504 $aIncludes bibliographical references and index.
505 00 $tNucleic acid transfer using cationic lipids /$rNatasha J. Caplen --$tOptimizing polyethylenimine-based gene transfer into mammalian brain for analysis of promoter regulation and protein function /$rBarbara A. Demeneix, Mohamed Ghorbel, Daniel Goula --$tGene transfer and drug delivery by electronic pulse delivery : a nonviral delivery approach /$rXi Zhao --$tStrategies for improving the frequency and assessment of homologous recombination /$rNancy Smyth Templeton --$tEffective gene transfer using viral vectors based on SV40 /$rDavid S. Strayer --$tRapid generation of isogenic mammalian cell lines expressing recombinant transgenes by use of cre recombinase /$rBrude D. Bethke, Brian Sauer --$tSite-directed alteration of genomic DNA by small-fragment homologous replacement /$rKaarin K. Goncz, Dieter C. Gruenert --$tMutation correction by homologous recombination with an adenovirus vector /$rAyumi Fujita-Kusano [and others] --$tSite-specific targeting of DNA plasmids to chromosome 19 using AAV cis and trans sequences /$rSamuel M. Young, Jr., Weidong Xiao, Richard Jude Samulski --$tAdeno-associated virus based gene therapy in skeletal muscle /$rRichard J. Bartlett, Jesica M. McCue --$tRapid establishment of myeloma cell lines expressing fab(tac)-protamine, a targetable protein vector, directed against high-affinity [alpha]-chain of human interleukin-2 receptor /$rSun U. Song, Wayne A. Marasco --$tEBV-derived episomes to probe chromatin structure and gene expression in human cells /$rJuliette Fivaz [and others] --$tTriplex-directed site-specific genome modification /$rKaren M. Vasquez, John H. Wilson --$tUse of quantitative ligation-mediated polymerase chain reaction to detect gene targeting by alkylating oligodeoxynucleotides /$rHoward B. Gamper [and others] --$tGene targeting in plants via site-directed mutagenesis /$rPeter B. Kipp [and others] --$tAntisense oligonucleotides as modulators of pre-mRNA splicing /$rHalina Sierakowska, Sudhir Agrawal, Ryszard Kole.
588 0 $aPrint version record.
520 $aIn Gene Targeting Protocols, a panel of innovative investigators present in readily reproducible detail the latest techniques for gene replacement, gene knockout, and gene repair in both animals and plants. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), discuss the parameters that affect vector uptake, and detail successful methods for gene targeting (Cre-ox, and adeno and adeno-associated viruses). The methods include two fundamentally different approaches to gene targeting- one using a fusion protein to deliver a vector to a virus for possible therapeutic effect, and one entailing an episomal-based Epstein-Barr vector that can modulate the chromatin assembly process, and thus overcome a serious barrier to therapeutic gene targeting. The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may revolutionize the entire field. Innovative and wide ranging, Gene Targeting Protocols offers today's researchers robust and proven gene-targeting techniques that are essential to understanding and regulating biological processes at the genetic level.
546 $aEnglish.
650 0 $aGene targeting$vLaboratory manuals.
650 0 $aGene targeting.
650 0 $aGenetics$xTechnique.
650 2 $aLaboratory Manuals
650 2 $aGene Targeting
650 2 $aGenetic Techniques
650 2 $aPublication Formats
650 2 $aInvestigative Techniques
650 2 $aPublication Characteristics
650 2 $aAnalytical, Diagnostic and Therapeutic Techniques and Equipment
650 4 $aGenetics.
650 4 $aBiology.
650 4 $aHealth & Biological Sciences.
650 6 $aGènes$xCiblage$vManuels de laboratoire.
650 6 $aGènes$xCiblage.
650 6 $aGénétique$xTechnique.
650 7 $aSCIENCE$xLife Sciences$xMolecular Biology.$2bisacsh
650 7 $aGenetics$xTechnique.$2fast$0(OCoLC)fst00940145
650 7 $aGene targeting.$2fast$0(OCoLC)fst00939637
655 2 $aLaboratory Manual
655 4 $aElectronic books.
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
655 7 $aLaboratory manuals.$2lcgft
655 7 $aManuels de laboratoire.$2rvmgf
700 1 $aKmiec, Eric B.
773 0 $tSpringer Protocols
776 08 $iPrint version:$tGene targeting protocols.$dTotowa, N.J. : Humana Press, ©2000$w(DLC) 99010333$w(OCoLC)40674932
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 133.
856 40 $uhttp://www.columbia.edu/cgi-bin/cul/resolve?clio4242788$zAll EBSCO eBooks
880 80 $6505-00/(S$g9.$tSite-specific targeting of DNA plasmids to chromosome 19 using AAV cis and trans sequences /$rSamuel M. Young Jr., Weidong Xiao & Richard Jude Samulski --$g10.$tAdeno-associated virus based gene therapy in skeletal muscle /$rRichard J. Bartlett & Jesica M. McCue --$g11.$tRapid establishment of myeloma cell lines expressing fab(tac)-protamine, a targetable protein vector, directed against high-affinity α-chain of human interleukin-2 receptor /$rSun U. Song & Wayne A. Marasco --$g12.$tEBV-derived episomes to probe chromatin structure and gene expression in human cells /$rJuliette Fivaz, M. Chiara Bassi, Stéphane Pinaud, Larry Richman, Melanie Price & Jovan Mirkovitch --$g13.$tTriplex-directed site-specific genome modification /$rKaren M. Vasquez & John H. Wilson --$g14.$tUse of quantitative ligation-mediated polymerase chain reaction to detect gene targeting by alkylating oligodeoxynucleotides /$rHoward B. Gamper, Irina Afonina, Evgeniy Belousov, Michael W. Reed & Mikhail A. Pdyminogin --$g15.$tGene targeting in plants via site-directed mutagenesis /$rPeter B. Kipp, Joyce M. Van Eck, Peter R. Beetham & Gregory D. May --$g16.$tAntisense oligonucleotides as modulators of pre-mRNA splicing /$rHalina Sierakowska, Sudhir Agrawal & Ryszard Kole.
852 8 $blweb$hEBOOKS