MARC Record from marc_columbia

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024 7 $a10.1385/1592592139.$2doi
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049 $aZCUA
245 00 $aImmunocytochemical methods and protocols /$cedited by Lorette C. Javois.
250 $a2nd ed.
260 $aTotowa, NJ :$bHumana Press,$c©1999.
300 $a1 online resource (xii, 465 pages) :$billustrations
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
490 1 $aMethods in molecular biology ;$vv. 115
504 $aIncludes bibliographical references and index.
505 00 $tPart I. Antibody Preparation --$tOverview of Antibody Use in Immunocytochemistry /$rSu-Yau Mao, Lorette C. Javois, and Ute M. Kent --$tPurification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration /$rUte M. Kent --$tPurification of Antibodies Using Ion-Exchange Chromatography /$rUte M. Kent --$tPurification of Antibodies Using Affinity Chromatography /$rUte M. Kent --$tPurification of Antibodies Using Protein A-Sepharose and FPLC /$rUte M. Kent --$tConjugation of Fluorochromes to Antibodies /$rSu-Yau Mao --$tBiotinylation of Antibodies /$rSu-Yau Mao --$tPart II. Tissue Preparation for light Microscopic Analyses --$tOverview of Cell Fixatives and Cell Membrane Permeants /$rMelissa A. Melan --$tPreparation of Frozen Sections for Analysis /$rGary L. Bratthauer --$tProcessing of Cytological Specimens /$rGary L. Bratthauer --$tProcessing of Tissue Culture Cells /$rGary L. Bratthauer --$tProcessing of Tissue Specimens /$rGary L. Bratthauer --$tAntigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections /$rLaszlo Krenacs, Tibor Krenacs, and Mark Raffeld --$tPart III. Light Microscopic Detection Systems --$tOverview of Fluorochromes /$rJ. Michael Mullins --$tDirect Immunofluorescent Labeling of Cells /$rLorette C. Javois --$tFluorescence Labeling of Surface Antigens on Attached or Suspended Tissue-Culture Cells /$rMark C. Willingham --$tFluorescence Labeling of Intracellular Antigens in Attached or Suspended Tissue-Culture Cells /$rMark C. Willingham --$tFluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts /$rLorette C. Javois --$tTUNEL Assay for Apoptotic Cells /$rVirginia M. Heatwole --$tOverview of Fluorescence Analysis with the Confocal Microscope /$rLiana Harvath --$tOverview of Laser Microbeam Applications as Related to Antibody Targeting /$rP. Scott Pine --$tOverview of Fluorescence Photomicography /$rLorette C. Javois and J. Michael Mullins --$tOverview of Antigen Detection Through Enzymatic Activity /$rGary L. Bratthauer --$tThe Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods /$rGary L. Bratthauer --$tThe Avidin Biotin Complex (ABC) Method and Other Avidin Biotin Binding Methods /$rGary L. Bratthauer --$tAvidin Biotin Labeling of Cellular Antigens in Cryostat Sectioned Tissue /$rMark Raffeld and Elaine S. Jaffe --$tMultiple Antigen Immunostaining Procedures /$rLaszlo Krenacs, Tibor Krenacs, and Mark Raffeld --$tELISA on Attachment Dependent or Suspension Grown Cells /$rJulie Ann Brent --$tUse of Immunogold with Silver Enhancement /$rConstance Oliver --$tPart IV. Fluorescence Activated Cell Sorter (FACS) Analyses --$tOverview of Flow Cytometry and Fluorescent Probes for Cytometry /$rRobert E. Cunningham --$tTissue Disaggregation/ Robert E. Cunningham --$tIndirect Immunofluorescent Labeling of Viable Cells /$rRobert E. Cunningham --$tIndirect Immunofluorescent Labeling of Fixed Cells /$rRobert E. Cunningham --$tFluorescent Labeling of DNA /$rRobert E. Cunningham --$tDeparaffinization and Processing of Pathologic Material /$rRobert E. Cunningham --$tAssay for Phagocytosis /$rLiana Harvath and Douglas A. Terle --$tAssay for Filamentous Actin /$rLiana Harvath --$tAssay for Chemoattractant Binding /$rLiana Harvath, Robert R. Aksamit, and Robert E. Cunningham --$tAssay for Oxidative Metabolism /$rLiana Harvath and Douglas A. Terle --$tPart V. Colloidal Gold Detection Systems For Electron Microscopic Analyses --$tFixation and Embedding /$rConstance Oliver and Maria Celia Jamur --$tPreparation of Colloidal Gold /$rConstance Oliver --$tConjugation of Colloidal Gold to Proteins /$rConstance Oliver --$tColloidal Gold/Streptavidin Methods /$rConstance Oliver --$tPre-Embedding Labeling Methods /$rConstance Oliver --$tPostembedding Labeling Methods /$rConstance Oliver --$tPart VI. Applications For Nucleic Acid Localization By In Situ Hybridization --$tOverview of In Situ Hybridization /$rRoland M. Nardone --$tIn Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe /$rRoland M. Nardone --$tFluorescence In Situ Hybridization Using Whole Chromosome Library Probes /$rRoland M. Nardone --$tIn Situ RT-PCR and Hybridization Techniques /$rVishakha Thaker --$tPart VII. Special Applications For the Clinical Laboratory --$tOverview of the Clinical Immunohistochemistry Laboratory : Regulations and Troubleshooting Guidelines /$rPatricia A. Fetsch and Andrea Abati --$tOverview of Immunohistochemical Approaches to the Differential Diagnosis of Tumors /$rDennis M. Frisman --$tOverview of Automated Immunostainers /$rGilbert E. Herman, Edna A. Elfont, and Alton D. Floyd.
588 0 $aPrint version record.
520 $aIn Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases.
546 $aEnglish.
650 0 $aImmunocytochemistry$vLaboratory manuals.
650 0 $aIn situ hybridization$vLaboratory manuals.
650 12 $aImmunohistochemistry$xmethods
650 12 $aImmunohistochemistry$xstandards
650 6 $aImmunocytochimie$vManuels de laboratoire.
650 6 $aHybridation in situ$vManuels de laboratoire.
650 7 $aSCIENCE$xLife Sciences$xAnatomy & Physiology.$2bisacsh
650 7 $aImmunocytochemistry.$2fast$0(OCoLC)fst00967934
650 7 $aIn situ hybridization.$2fast$0(OCoLC)fst00968339
650 17 $aImmunocytochemie.$2gtt
650 17 $aWetenschappelijke technieken.$2gtt
653 00 $abiochemie
653 00 $abiochemistry
653 00 $aimmunocytochemie
653 00 $aimmunocytochemistry
653 10 $aBiology (General)
653 10 $aBiologie (algemeen)
655 2 $aLaboratory Manual
655 4 $aElectronic books.
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
655 7 $aGidsen (vorm)$2gtt
655 7 $aLaboratory manuals.$2lcgft
655 7 $aManuels de laboratoire.$2rvmgf
700 1 $aJavois, Lorette C.
776 08 $iPrint version:$tImmunocytochemical methods and protocols.$b2nd ed.$dTotowa, NJ : Humana Press, ©1999$z0896038130$w(DLC) 98046930$w(OCoLC)40200663
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 115.
856 40 $uhttp://www.columbia.edu/cgi-bin/cul/resolve?clio4244210$zAll EBSCO eBooks
852 8 $blweb$hEBOOKS