MARC Record from marc_columbia

LEADER: 04153mam a2200397 a 4500
001 4048231
005 20221027024321.0
008 950515t19951995enka b 000 0 eng d
010 $agb 95038213
015 $aGB95-38213
020 $a0131231189
035 $a(OCoLC)490627575
035 $a(OCoLC)ocn490627575
035 $9AKS9306HS
035 $a(NNC)4048231
035 $a4048231
040 $aERM$bfre$cERM$dUKM$dNNC-M$dOrLoB
245 00 $aReverse transcriptase PCR /$cedited by James W. Larrick and Paul D. Siebert.
260 $aLondon ;$aNew York ;$aToronto :$bEllis Horwood,$c[1995], ©1995.
300 $aviii, 346 pages :$billustrations ;$c25 cm.
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
490 1 $aEllis Horwood series in pharmaceutical technology
504 $aIncludes bibliographical references.
505 0 $a1. Introduction and overview / Paul D. Siebert and James W. Larrick -- 2. RT-PCR on single cell after patch clamp recording / Bertrand Lambolez, Etienne Audinet, Pascal Bochet and Jean Rossier -- 3. Reducing false positives with RNA template-specific PCR (RS-PCR) / Alan R. Shuldiner and Ze Huang -- 4. Detection and analysis of T cell receptor gene products by RT-PCR / Jorge R. Oksenberg and Lawrence Steinman -- 5. RT in situ PCR for detection of viral and mRNAs / Phyllis MacConnell and Gerard J. Nuovo -- 6. A combined RT-PCR/Southern hybridization strategy and its application to homeotic mRNA expression in circulating CD34[superscript +] pluripotent stem cells / Christopher S. Hayes, Kenneth A. Ault and Calvin P. H. Vary -- 7. Evaluation of absolute mRNA levels by polymerase chain reaction-aided transcript titration assay (PATTY) / Michael Becker-Andre -- 8. Limiting dilution polymerase chain reaction / Pamela J. Sykes and Alexander A. Morley.
505 8 $a9. Quantitative measurement of relative gene expression in human tumors / Heinz-Josef Lenz, Kathleen D. Danenburg and Peter V. Danenburg -- 10. Quantitative RT-PCR based on multiplex amplification curves / Tomohiro Kinoshita and Kunitada Shimotohno -- 11. [alpha]1 and [alpha]2 type IV collagen mRNAs in adult mouse and human glomeruli: quantitation by competitive PCR / Emmanuel P. Peten, Liliane J. Striker and Gary E. Striker -- 12. Ligation-independent cloning of PCR products / Randy S. Haun -- 13. Anchor ligated cDNA libraries: a technique for generating a cDNA library for the immediate cloning of the 5 ends of mRNAs / Aaron N. Apte and Paul D. Siebert -- 14. Equalized cDNA libraries / Minoru S. H. Ko -- 15. cDNA selection with cosmids and YACs / Satish Parimoo -- 16. Semi-quantitative reverse transcriptase-polymerase chain reaction for the detection of low abundance transcripts in human cells / Mei He, Edison Liu and Kathleen Conway.
505 8 $a17. Detection of p53 gene mutations by RT-PCR-SSCP / Atsushi Ichikawa, Tomoriro Kinoshita, Tomomitsu Hotta and Hidehiko Saito -- 18. SSCP analysis of the PCR product of RB cDNA / Yoshinori Murakami and Takao Sekiya -- 19. The application of RT-PCR to other infectious diseases / Zayd A. Eldadah and David M. Asher.
520 $aRT-PCR is now a fundamental tool for biological and biomedical research and diagnostics. This unique book, devoted to the real-life applications of the polymerase chain reaction (PCR) methodology, takes a trouble-shooting approach. It is both timely and topical, reflecting the immense experience of the authors, regarded as pioneers in this field of research.
520 8 $aUnlike many existing texts on the topic, it is up-to-date and pragmatic, and will be well received by research communities and industrial researchers alike.
650 2 $aPolymerase Chain Reaction.$0https://id.nlm.nih.gov/mesh/D016133
650 2 $aRNA-Directed DNA Polymerase.$0https://id.nlm.nih.gov/mesh/D012194
650 2 $aRNA.$0https://id.nlm.nih.gov/mesh/D012313
700 1 $aLarrick, James W.$0http://id.loc.gov/authorities/names/n85321396
700 1 $aSiebert, Paul D.$0http://id.loc.gov/authorities/names/nr95033096
830 0 $aEllis Horwood series in pharmaceutical technology.
852 00 $boff,hsl$hQP606.R48$iR48 1995