MARC Record from marc_columbia

LEADER: 03359cam a22003373i 4500
001 10974130
005 20180618182641.0
006 m o d
007 cr |n||||a||||
008 141006s2013 nyu|||| om 00| ||eng d
035 $a(OCoLC)893604443
035 $a(OCoLC)ocn893604443
035 $a(NNC)ACfeed:legacy_id:ac:177562
035 $a(NNC)ACfeed:doi:10.7916/D8HX1M18
035 $a(NNC)10974130
040 $aNNC$beng$erda$cNNC
100 1 $aKim, Jinho.
245 12 $aA Microfluidic Approach to Selection and Enrichment of Aptamers for Biomolecules and Cells /$cJinho Kim.
264 1 $a[New York, N.Y.?] :$b[publisher not identified],$c2013.
300 $a1 online resource.
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
502 $aThesis (Ph.D.)--Columbia University, 2013.
500 $aDepartment: Mechanical Engineering.
500 $aThesis advisor: Qiao Lin.
520 $aThis thesis presents microfluidic devices for selection and amplification of nucleic acids (aptamers) that bind to specific targets. Aptamers are very attractive molecules in many biological applications due to their interesting properties including high target binding affinities and stability. Using conventional platforms for aptamer generation (SELEX, systematic evolution of ligands by exponential enrichment) is labor-intensive and time consuming. Microfluidic devices have been developed to improve the aptamer enrichment efficiency. However, aptamer generation using these devices is still inefficient because they require complicated flow control components for sample and reagent handling and additional off-chip processes. We developed microfluidic SELEX platforms for rapid isolation of aptamers that possess greatly simplified designs which enable easy chip fabrication and operation. The simplicity of the devices is achieved by utilizing a combination of bead-based selection and amplification of target binding nucleic acids, and gel-based electrokinetic transfer of nucleic acids.
520 $aIn the devices, nucleic acids that bind to targets are isolated on target-functionalized microbeads or target cells in a microchamber and electrokinetically transported to another chamber through a gel-filled microchannel by an electric field. The strands are then hybridized onto reverse primers immobilized on microbeads and amplified via polymerase chain reaction (PCR) using on-chip temperature control. The amplified strands are separated from the beads and electrophoretically transferred back into the selection chamber for subsequent SELEX rounds. Using the devices, we demonstrated enrichment of target-binding nucleic acids against human immunoglobulin E (IgE), the glucose-boronic acid complex, and MCF-7 cancer cells. With the physical and functional integration allowed by the monolithic design realized in our devices, the total process time for selection of aptamers was drastically reduced compared with that required by conventional aptamer selection platforms. Moreover, the binding affinities of the selected strands using our devices are comparable to those of aptamers obtained using the conventional platforms.
653 0 $aMechanical engineering
856 40 $uhttps://doi.org/10.7916/D8HX1M18$zClick for full text
852 8 $blweb$hDISSERTATIONS