MARC Record from Library of Congress

LEADER: 02174cam a22003254a 4500
001 2005022077
003 DLC
005 20070422080604.0
008 050729s2006 nyua b 001 0 eng
010 $a 2005022077
020 $a0879697725 (hardcover : alk. paper)
020 $a0879697717 (pbk. : alk. paper)
020 $a9780879697716
035 $a(OCoLC)ocm61253855
040 $aDNLM/DLC$cDLC$dBAKER$dC#P$dUBY$dCOO$dBTCTA$dYDXCP$dDLC
042 $apcc
050 00 $aQH442.2$b.S24 2006
082 00 $a572.8$222
100 1 $aSambrook, Joseph.
245 14 $aThe condensed protocols from Molecular cloning : a laboratory manual /$cJoseph Sambrook, David W. Russell.
260 $aCold Spring Harbor, N.Y. :$bCold Spring Harbor Laboratory Press,$cc2006.
300 $av, 800 p. :$bill. ;$c28 cm.
500 $a"A single-volume adaptation of the three-volume third edition of Molecular cloning: a laboratory manual"--P. [4] of cover.
504 $aIncludes bibliographical references and index.
505 0 $aPlasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
650 0 $aMolecular cloning$vLaboratory manuals.
650 2 $aCloning, Molecular$vLaboratory Manuals.
700 1 $aRussell, David W.$q(David William),$d1957-
700 1 $aSambrook, Joseph.$tMolecular cloning.
856 41 $3Table of contents$uhttp://www.loc.gov/catdir/toc/ecip0517/2005022077.html