MARC Record from Library of Congress

LEADER: 04332cam a22004697i 4500
001 2015934691
003 DLC
005 20151103090559.0
008 150226t20152015nyua b 001 0 eng
010 $a 2015934691
016 7 $a101662880$2DNLM
020 $a9781493924974 (alk. paper)
020 $a1493924974 (alk. paper)
020 $z9781493924981 (ebk.)
020 $z1493924982 (ebk.)
024 7 $a10.1007/978-1-4939-2498-1$2doi
035 $a(OCoLC)ocn899226184
040 $aNLM$beng$cNLM$erda$dYDXCP$dBTCTA$dHNK$dNUI$dOCLCO$dDLC
042 $anlmcopyc$alccopycat
050 00 $aQR185.8.T2$bT353 2015
060 00 $aW1$bME9616J v.1291 2015
060 10 $aWH 25
082 00 $a616.07/97$223
245 00 $aT follicular helper cells :$bmethods and protocols /$cedited by Marion Espéli, INSERM UMR_S996, LabEx LERMIT, Université Paris-Sud, Clamart, France, Michelle Linterman, Lymphocyte Signalling and Development ISP, Babraham Institute, Babraham Research Campus, Cambridge, UK.
264 1 $aNew York, NY :$bHumana Press,$c[2015]
300 $axi, 228 pages :$billustrations (some color) ;$c27 cm.
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
490 1 $aMethods in molecular biology,$x1064-3745 ;$v1291
490 1 $aSpringer protocols,$x1949-2448
504 $aIncludes bibliographical references and index.
505 0 $aIdentification of Mouse T Follicular Helper Cells by Flow Cytometry -- Identification of Follicular T Helper Cells in Tissue Sections -- Tracking Early T Follicular Helper Cell Differentiation In vivo -- Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination -- Retroviral Vector Expression in TCR Transgenic CD4+ T Cells -- Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells -- In vivo Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function -- Assessing T Follicular Helper Cell Function in vivo: Antigen-specific B Cell Response to Hapten and Affinity Maturation -- The SWHEL System For High-resolution Analysis of in vivo Antigen-specific T-Dependent B Cell Responses -- Triggering Positive Selection of Germinal Center B cells by Antigen Targeting to DEC-205 -- Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry -- Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry -- In vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell Responses -- Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells -- Human Tfh and Tfr cells: Identification and Assessment of their Migration Potential -- Analysis of Human Blood Memory T Follicular Helper Subsets -- Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood -- Quantifying Helper Cell Function of Human TFH Cells in vitro.
520 $aThis volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward. --$csource other than Library of Congress.
650 0 $aTh1 cells$xPhysiological effect$vLaboratory manuals.
650 0 $aTh2 cells$xPhysiological effect$vLaboratory manuals.
650 0 $aT cells$xPhysiological effect$vLaboratory manuals.
650 12 $aT-Lymphocytes, Helper-Inducer$vLaboratory Manuals.
700 1 $aEspéli, Marion,$eeditor.
700 1 $aLinterman, Michelle,$eeditor.
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 1291.$x1064-3745
830 0 $aSpringer protocols (Series)$x1949-2448