MARC Record from Library of Congress

LEADER: 04444cam a22003857a 4500
001 2011928685
003 DLC
005 20120125172601.0
008 110502s2011 nyua a 001 0 eng d
010 $a 2011928685
020 $a1617791253
020 $a9781617791253
020 $a9781617791260 (e-isbn)
020 $a1617791261 (e-isbn)
035 $a(OCoLC)ocn706784502
040 $aYDXCP$cYDXCP$dRML$dCUS$dBWX$dCOO$dCDX$dDLC
042 $alccopycat
050 00 $aQH603.C43$bR42 2011
082 00 $a572/.696$223
245 00 $aReceptor signal transduction protocols /$cedited by Gary B. Willars and R.A. John Challiss.
250 $a3rd ed.
260 $aNew York :$bSpringer,$cc2011.
300 $axi, 479 p. :$bill. (some col.) ;$c27 cm.
490 1 $aSpringer protocols
490 1 $aMethods in molecular biology,$x1064-3745 ;$v746
504 $aIncludes bibliographical references and index.
505 00 $tInducible expression of G protein-coupled receptors in transfected cells /$rBeryl Koener and Emmanuel Hermans --$tUsing the Flp-In T-Rex system to regulate GPCR expression /$rRichard J. Ward, Elisa Alvarez-Curto, and Graeme Milligan --$tViral infection for GPCR expression in eukaryotic cells /$rAntonio Porcellini, Luisa Iacovelli, and Antonio De Blasi --$tGeneration of epitope-tagged GPCRs /$rYan Huang and Gary B. Willars --$tUse of site-directed mutagenesis to study GPCRs /$rAlex C. Conner ... [et al.] --$tApproaches to study GPCR regulation in native systems /$rJonathon M. Willets --$tHeterologous expression of GPCRs in fission yeast /$rJohn Davey and Graham Ladds --$tRadioligand binding methods for membrane preparations and intact cells /$rDavid B. Bylund and Myron L. Toews --$tQuantification of GPCR mRNA using real-time RT-PCR /$rTrond Brattelid and Finn Olav Levy --$tDetermining allosteric modulator mechanism of action : integration of radioligand binding and functional assay data /$rChristopher J. Langmead --$tDesign and use of fluorescent ligands to study ligand-receptor interactions in single living cells /$rStephen J. Briddon, Barrie Kellam, and Stephen J. Hill --$tExamining site-specific GPCR phosphorylation /$rAdrian J. Butcher, Andrew B. Tobin, and Kok Choi Kong --$tUbiquitination of GPCRs /$rAdriana Caballero and Adriano Marchese --$t[35S]GTPgS binding as an index of total G-protein and Ga-subtype-specific activation by GPCRs /$rRajendra Mistry, Mark R. Dowling, and R. A. John Challiss --$tUsing calcium imaging as a readout of GPCR activation /$rMartin D. Bootman and H. Llewelyn Roderick --$tMeasuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors /$rFrank Gesellchen ... [et al.] --$tDetermining the activation of Rho as an index of receptor coupling to G12/13 proteins /$rMichio Nakaya ... [et al.] --$tUse of translocating fluorescent biosensors for real-time monitoring of GPCR-mediated signaling events /$rCarl P. Nelson and R.A. John Challiss --$tStudy of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs /$rAshley E. Brady ... [et al.] --$tStudy of GPCR-protein interactions by BRET /$rMartina Kocan and Kevin D.G. Pfleger --$tTime resolved FRET strategy with fluorescent ligands to analyze receptor interactions in native tissues : application to GPCR oligomerization /$rMartin Cottet ... [et al.] --$tPeptide affinity purification for the isolation and identification of GPCR-associated protein complexes /$rPascal Maurice, Avais M. Daulat, and Ralf Jockers --$tTandem affinity purification and identification of GPCR-associated protein complexes /$rAvais M. Daulat, Pascal Maurice, and Ralf Jockers --$tIdentification of GPCR localization in detergent resistant membranes /$rRanju Kumari and Anna Francesconi --$tAnalysis of GPCR localization and trafficking /$rJames N. Hislop and Mark von Zastrow --$tStatistical methods in research /$rDomenico Spina.
650 0 $aCell receptors$xResearch$xMethodology.
650 0 $aCellular signal transduction$xResearch$xMethodology.
650 2 $aSignal Transduction$xphysiology$vLaboratory Manuals.
700 1 $aWillars, Gary B.
700 1 $aChalliss, R. A. J.$q(R. A. John)
776 08 $iOnline version:$tReceptor signal transduction protocols.$b3rd ed.$dNew York : Humana Press, c2011$z9781617791260$w(OCoLC)730973427
830 0 $aSpringer protocols.
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$vv. 746.