MARC Record from University of Toronto

LEADER: 02169nam 2200253 4500
001 AAIMR02448
005 20060407065114.5
008 060407s2005 onc|||||||||||||| ||eng d
020 $a0494024488
039 $fws
100 1 $aGibbs, Jason.
245 10 $aSynapsis and meiotic nodules in the Lilium species-hybrid "Black Beauty" prepared using a novel plant chromosome spread technique for ultra-structural and immunocytochemical analyses.
260 $c2005.
300 $a110 leaves.
500 $aSource: Masters Abstracts International, Volume: 44-01, page: 0232.
502 $aThesis (M.Sc.)--University of Toronto, 2005.
506 $aElectronic version licensed for access by U. of T. users.
520 $aA method for spreading meiotic chromosomes was developed for examining synaptonemal complex (SC) formation and meiotic nodules (MN) in the Lilium species-hybrid Black Beauty. Preliminary studies suggest this technique has utility for EM immunocytochemical analyses. Black Beauty only completes 35.2% synapsis. The axial length in pachytene microsporocytes was 3699.5mum in Black Beauty and 4492.2mum in the control L. longiflorum cv. Nellie White. The number of potential synaptic initiations was estimated to be 528 for Black Beauty and 668 for Nellie White. The species-hybrid was estimated to successfully complete only 243 synaptic initiations. The synaptic defect is believed to result from an inability to convert axial associations into SC formation due to failed homology checks. Analysis of MN frequency on the chromosome axis found no difference between Black Beauty and Nellie White. MNs appeared at synaptic forks in higher than expected frequencies. The possible relationship of MNs to synapsis is discussed.
590 $aROBARTS MICROTEXT copy on microfiche.$5CaOTU
653 $aBiology, Molecular.
653 $aBiology, Botany.
856 41 $uhttp://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=369933&T=F$yConnect to resource
949 $atheses masters$wASIS$c1$i5859148-1001$lINPROCESS$mROBARTS$rN$sY$tMICROFORM$u1/5/2006
949 $aOnline resource 369933$wASIS$c1$i5859148-2001$lONLINE$mE_RESOURCE$rY$sY$tE_RESOURCE$u1/5/2006