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HPLC methods for clinical pharmaceutical analysis: a user's guide
2012, Wiley-VCH
in English
- 1st ed.
3527331298 9783527331291
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Book Details
Table of Contents
1.Introduction
1.1.First Question: Determination of Ibuprofen in Plasma
1.2.Second Question: Determination of Tryptophan in Urine
1.3.Third Question: Determination of Paclitaxel in Tissue
2.Planning of Analyses
2.1.Introduction
2.2.Limit of Detection (LOD) and Determination (LLOQ)
2.3.Detectors
2.4.Structure of the Analyte
2.5.Solubility of an Analyte
2.6.Selection of the Detector
3.Sample Preparation
3.1.Dilution
3.2.Protein Precipitation, Overview
3.3.Extraction
4.HPLC Separation
4.1.HPLC Pumps
4.2.Degasser
4.3.Injector
4.4.HPLC Columns
5.Detection
5.1.Detection in the Pharmaceutical/Bioanalytical Area
5.2.Detection in the "Clinical Area" (Therapy Control/Compliance)
6.Chemical Derivatization for Detection Enhancement
7.Validation Concepts
7.1.Introduction
7.2.Realization of the FDA Guideline
8.Practical Hints Concerning Stability, Destruction and Degradation Products
9.Metabolites
10.Internal Standards
11.Case Studies with Intensive Discussion for Each Substance
11.1.Acetylcarnitine in Plasma
11.2.Acetylcysteine in Plasma
11.3.Acyclovir in Plasma and Urine
11.4.Caffeine in Plasma
11.5.Diazepam in Plasma
11.6.Diclofenac in Plasma
11.7.Dihydralazine in Plasma
11.8.Duramycin (Moli1901) in Plasma
11.9.Fluticasone Propionate in Plasma
11.10.Hydroxytriamterene Sulfate and Triamterene in Plasma and Urine
11.11.Ibuprofen in Plasma (also Enantiomeric Separation)
11.12.Minocycline in Plasma
11.13.Norfloxacine in Plasma and Urine
11.14.Paclitaxel in Plasma, Urine and Tissue
11.15.Paracetamol (Acetaminophen) in Plasma
11.16.Pimelic Acid in Plasma and Urine
11.17.8-Prenylnaringenin in Plasma and Different Types of Tissues
11.18.Silibinin in Plasma
11.19.Valnemulin in Plasma, Different Tissue Types and in Animal Feed
11.20.Vitamin B1 (Total Thiamine) in Plasma
Table 11.1 Recommended books
Table 11.2 Properties of substances
Appendix
Short Description of Determination for about 100 Substances
Substances listed in the Appendix.
Edition Notes
Includes bibliographical references and index.
Classifications
The Physical Object
ID Numbers
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