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1
Generation of CDNA Libraries: Methods and Protocols
2003, Humana Press
in English
1592593593 9781592593590
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2
Generation of cDNA libraries: methods and protocols
2003, Humana Press
in English
1588290662 9781588290663
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Book Details
Table of Contents
Complementary DNA libraries: an overview / Shao-Yao Ying
Rapid amplification of cDNA ends / Yue Zhang
cDNA generation on paramagnetic beads / Zhaohui Wang and Michael G.K. Jones
Construction of a normalized cDNA library by mRNA-cDNA hybridization and subtraction / Ye-Guang Chen
Amplification of cDNA ends using PCR suppression effect and step-out PCR / Mikhail V. Matz ... [et al.]
Use of inverse PCR to clone cDNA ends / Sheng-He Huang, Steven H.M. Chen, and Ambrose Y. Jong
Construction of size-fractionated cDNA library assisted by an in vitro recombination reaction / Osamu Ohara
Construction of a full-length enriched and 5'-end enriched cDNA library using the oligo-capping method / Yutaka Suzuki and Sumio Sugano
cDNA library construction using in vitro transcriptional amplification / Shi-Lung Lin and Henry Ji
Amplification of representative cDNA pools from microscopic amounts of animal tissue / Mikhail V. Matz
Single cell cDNA library construction using cycling aRNA amplification / Shi-Lung Lin
mRNA/cDNA library construction using RNA-polymerase cycling reaction / Shi-Lung Lin and Shao-Yao Ying
Quality assessment of cDNA libraries / Hans-Jürgen Fülle
Assessment of the quality of mRNA libraries by agarose gel electrophoresis / Tsen-Yin Lin and Shao-Yao Ying
PACS RT-PCR: a method for the generation and measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA / Igor Nepluev and Rodney J. Folz
Single-cell mRNA library analysis by northern blot hybridization / Shi-Lung Lin
Generation of cDNA libraries for profiling gene expression of given tissues or cells / Xin Zhang, Qiu-Hua Huang, and Ze-Guang Han
Screening poly (dA/dT(-)) cDNA for gene identification / San Ming Wang ... [et al.]
Generation of longer cDNA fragments from SAGE tags for gene identification / Jian-Jun Chen ... [et al.]
Generation of full-length cDNA libraries enriched for differentiatlly expressed genes / Bakhyt Zhumabayeva ... [et al.]
Subtractive hybridization for the identification of differentially expressed genes using uracil-DNA glycosylase and mung-bean nuclease / Tsen-Yin Lin and Shao-Yao Ying
Subtractive cloning of differential genes using RNA-PCR / Shao-Yao Ying and Shi-Lung Lin
Strategy for construction of a cDNA encoding a repetitive amino acid sequence / Masahiro Asada and Toru Imamura
Preparing lambda libraries for expression of proteins in prokaryotes or eukaryotes / Rebecca L. Mullinax and Joseph A. Sorge
Peptide library construction from RNA-PCR-derived RNAs / Shi-Lung Lin
Identifying interacting proteins in an Escherichia coli-based two-hybrid system / Bonnie Wu, Rebecca L. Mullinax, and Joseph A. Sorge
Future perspectives / Shao-Yao Ying
Edition Notes
Includes bibliographical references and index
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