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Pharmaceutical scientists in industry and academia will appreciate this single reference for its detailed experimental procedures for conducting biopharmaceutical studies. This well-illustrated guide allows them to establish, validate, and implement commonly used in situ and in vitro model systems. Chapters provide ready access to these methodologies for studies of the intestinal, buccal, nasal and respiratory, vaginal, ocular, and dermal epithelium as well as the endothelial and elimination barriers.
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Models for Assessing Drug Absorption and Metabolism
1996, Springer US, Imprint, Springer
electronic resource /
in English
1489918639 9781489918635
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Table of Contents
1 General Principles in the Characterization and Use of Model Systems for Biopharmaceutical Studies
2 Methods for Evaluating Intestinal Permeability and Metabolism in Vitro
3 Cultured Intestinal Epithelial Cell Models
4 Intestinal Rings and Isolated Intestinal Mucosal Cells
5 Models of Drug Absorption in Situ and in Conscious Animals
6 Model Systems for Intestinal Lymphatic Transport Studies
7 Buccal Tissues and Cell Culture
8 Isolated Hepatocytes
9 Cultured Rat Hepatocytes
10 Isolated Perfused Liver
11 Isolated Renal Brush Border and Basolateral Membrane Vesicles and Cultured Renal Cells
12 Use of An Isolated Perfused Kidney to Assess Renal Clearance of Drugs: Information Obtained in Steady-State and Non-Steady-State Experimental Systems
13 Brain Microvessel Endothelial Cell Culture Systems
14 Methods to Study Drug Transport in Isolated Choroid Plexus Tissue and Cultured Cells
15 Brain Perfusion Systems for Studies of Drug Uptake and Metabolism in the Central Nervous System
16 In Vitro Nasal Models
17 Models for Investigation of Peptide and Protein Transport across Cultured Mammalian Respiratory Epithelial Barriers
18 Drug Transport across Xenopus Alveolar Epithelium in Vitro
19 In Situ and in Vivo Methods for Pulmonary Delivery
20 In Vitro Viable Skin Model
21 Isolated Perfused Porcine Skin Flap Systems
22 Vaginal Epithelial Models
23 Ocular Epithelial Models.
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