An edition of Sirtuins
(2013)
Sirtuins
methods and protocols
by Matthew D. Hirschey
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Subjects
Proteins, Sirtuins, Proteins, analysis| Edition | Availability |
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Book Details
Table of Contents
Introduction: sirtuins in aging and diseases -- Leonard Guarente
Sirtuins in yeast: phenotypes and tools -- Scott Tsuchiyama [and four others]
C. elegans sirtuins -- Mohan Viswanathan and Heidi A. Tissenbaum
Genetic and biochemical tools for investigating sirtuin function in Drosophila melanogaster -- Jason G. Wood, Rachel Whitaker, and Stephen L. Helfand
Generating mammalian sirtuin tools for protein-interaction analysis -- Kathleen A. Hershberger [and three others]
Mass spectrometry-based detection of protein acetylation -- Yu Li [and three others]
SILAC-based quantification of Sirt1-responsive lysine acetylome -- Yue Chen, Gozde Colak, and Yingming Zhao
Targeted quantitation of acetylated lysine peptides by selected reaction monitoring mass spectrometry -- Matthew J. Rardin, Jason M. Held, and Bradford W. Gibson
Identification of deacetylase substrates with the biotin switch approach -- J. Will Thompson, Alex Robeson, and Joshua L. Andersen
Assaying chromatin sirtuins -- Lei Zhong [and four others]
Measurement of sirtuin enzyme activity using a substrate-agnostic fluorometric nicotinamide assay -- Basil P. Hubbard and David A. Sinclair
Detecting sirtuin-catalyzed deacylation reactions using 32P-labeled NAD and thin-layer chromatography -- Anita Zhu, Xiaoyang Su, and Hening Lin
Chemical acetylation and deacetylation -- Kristofer S. Fritz
Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography -- Jun Yoshino and Shin-ichiro Imai
In vivo measurement of the acetylation state of sirtuin substrates as a proxy for sirtuin activity -- John Dominy, Pere Puigserver, and Carles Cantó
Oxygen flux analysis to understand the biological function of sirtuins -- Dongning Wang [and three others]
The emerging links between sirtuins and autophagy -- In Hye Lee, Jeanho Yun, and Toren Finkel
Methods to study the role of sirtuins in genome stability -- Paloma Martínez-Redondo and Alejandro Vaquero
Circadian measurements of sirtuin biology -- Kathryn Moynihan Ramsey [and five others]
Utilizing calorie restriction to evaluate the role of sirtuins in healthspan and lifespan of mice -- Jessica Curtis and Rafael de Cabo.
Edition Notes
Includes bibliographical references and index.
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| October 5, 2021 | Edited by ImportBot | import existing book |
| November 12, 2020 | Created by MARC Bot | import new book |