An edition of Protein Engineering
(2004)
Protein engineering
by Joseph P. Noel and Dan Robertson
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Previews available in: English
Subjects
Genetic engineering, Proteins, Protein engineering, Synthesis, Structure-activity relationships, Conformation, Genetic Engineering, Protein Conformation, Genetics, Protein Engineering, Protein Biosynthesis, Technologie des protéines, Protéines, Synthèse, Manipulations génétiques, Relations structure-activité, Génie génétique, Labortechnik, Proteindesign, DNA, Recombinatie, Eiwitsynthese, Ingénierie des protéines, Synthèse protéique, Manipulation génétique, Relation structure-activité (Biochimie), ProtéineShowing 2 featured editions. View all 2 editions?
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Book Details
Published in
Amsterdam, Boston
Table of Contents
Section I. Methodology
Gene site saturation mutagenesis : a comprehensive mutagenesis approach
Single-stranded DNA family shuffling
Module shuffling
SCHEMA-guided protein recombination
Staggered extension process in vitro DNA recombination
Using incremental truncation to create libraries of hybrid enzymes
Random multirecombinant polymerase chain reaction
Gene library synthesis by structure-based combinatorial protein engineering
New enzymes from combinatorial library modules
Construction of protein fragment complementation libraries using incremental truncation
Section II. Applications: optimization and screening
GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform
High throughput microplate screens for directed protein evolution
Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities
Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis
Screening for oxidative resistance
Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes
Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system
Section III. Applications: directed evolution of enzymatic function
Directed evolution of lipases and esterases
Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium
Directed evolution of aldolases
Changing the enantioselectivity of enzymes by directed evolution
Control of stereoselectivity in phosphotriesterase
Section IV. Applications: evolution of biosynthetic pathways
Manipulation and analysis of polyketide synthases
Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases
Engineering carotenoid biosynthetic pathways
Section V. Devices, antibodies, and vaccines
Directed evolution of bacteriorhodopsin for device applications
Engineering antibody affinity by yeast surface display
A conditionally replicating virus as a novel approach toward an HIV vaccine.
Edition Notes
Includes bibliographical references and indexes.
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| July 16, 2022 | Edited by ImportBot | import existing book |
| September 9, 2021 | Created by ImportBot | import existing book |