Buy this book
![Loading indicator](/images/ajax-loader-bar.gif)
Abstract: Clostridium perfringens iota toxin is a binary toxin composed of the enzymatically active component Ia and receptor binding component Ib. Ia is an ADP-ribosyltransferase, which modifies Arg177 of actin. The previously determined crystal structure of the actin-Ia complex suggested involvement of Asp179 of actin in the ADP-ribosylation reaction. To gain more insights into the structural requirements of actin to serve as a substrate for toxin-catalyzed ADP-ribosylation, we engineered Saccharomyces cerevisiae strains, in which wild type actin was replaced by actin variants with substitutions in residues located on the Ia-actin interface. Expression of the actin mutant Arg177Lys resulted in complete resistance towards Ia. Actin mutation of Asp179 did not change Ia-induced ADP-ribosylation and growth inhibition of S. cerevisiae. By contrast, substitution of Glu270 of actin inhibited the toxic action of Ia and the ADP-ribosylation of actin. In vitro transcribed/translated human β-actin confirmed the crucial role of Glu270 in ADP-ribosylation of actin by Ia
Buy this book
![Loading indicator](/images/ajax-loader-bar.gif)
Subjects
Clostridium perfringens, ADP-Ribosylierung, Actin, Krebs, OnkologieShowing 1 featured edition. View all 1 editions?
Edition | Availability |
---|---|
1
Roles of Asp179 and Glu270 in ADP-ribosylation of Actin by Clostridium perfringens Iota Toxin
2015, Universität
in English
|
aaaa
|
Book Details
Edition Notes
PLoS one. 10, 12 (2015), e0145708, DOI 10.1371/journal.pone.0145708, issn: 1932-6203
IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs
Archivierung/Langzeitarchivierung gewährleistet
Classifications
The Physical Object
ID Numbers
Community Reviews (0)
Feedback?April 26, 2022 | Created by MARC Bot | import new book |