An edition of The veterinary laboratory and field manual
(2002)
The veterinary laboratory and field manual
a guide for veterinary laboratory technicians and animal health advisors
2nd ed.
by S. C. Cork, Susan C. Cork, and Roy W. Halliwell
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Subjects
Laboratory Techniques and Procedures, Methods, Pathology, Veterinary, Veterinary, Veterinary clinical pathology, Veterinary laboratories, Veterinary pathology, Veterinary pathology & histology, Medical / Nursing, Medical, Agriculture - Animal Husbandry, Veterinary Medicine - Food Animal, Medical / Veterinary Medicine / General, VETERINARY MEDICINEShowing 3 featured editions. View all 3 editions?
| Edition | Availability |
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1
The veterinary laboratory and field manual: a guide for veterinary laboratory technicians and animal health advisors
2012, Nottingham University Press
in English
- 2nd ed.
1908062096 9781908062093
|
aaaa
Libraries near you:
WorldCat
|
2
Technical Veterinary Handbook: A Guide for Veterinary Laboratory Technicians and Animal Health Advisors
July 1, 2003, Nottingham University Press
Hardcover
in English
1897676492 9781897676493
|
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Libraries near you:
WorldCat
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3
The veterinary laboratory and field manual: a guide for veterinary laboratory technicians and animal health advisors
2002, Nottingham University Press
in English
1897676492 9781897676493
|
zzzz
Libraries near you:
WorldCat
|
Book Details
Table of Contents
Machine generated contents note:
1.
Setting Up And Using A Laboratory Service -- -- 1.1.
The role of the veterinary laboratory network within animal health extension services --
Infrastructure and function of the regional or district veterinary laboratory --
Legislation and responsibility --
Education and public relations --
Practical training (workshops) --
Field visits --
Planning field work and the use of a mobile laboratory --
Buildings and maintenance --
Location and design --
Services --
Power supply: Electricity and gas --
Water supply --
Transport and communication services --
Managing supplies --
Stock control --
Store sections --
A note on district laboratories -- -- 1.2.
Staff requirements --
Field work --
Laboratory --
Training laboratory technicians -- -- 1.3.
Safety in the laboratory --
Handling potentially infectious material --
Legislation (national and local authorities) --
Waste disposal and biosecurity --
Incinerators --
Biological pits --
Procedures and protocols --
Apparatus --
Glassware --
Pipettes --
Knives and sharp implements --
Chemicals --
Fire prevention and control -- -- 1.4.
Sample selection, submission and clinical diagnosis --
Clinical examination --
General guidelines for performing a clinical examination --
Sample submission, preservation and communication with the laboratory --
General guidelines for submitting samples to the laboratory --
Sample collection kits --
Collection of material for laboratory examination --
Specimens and laboratory diagnosis --
Sending specimens to the laboratory -- -- 1.5.
Quality assurance and control --
Providing a quality service --
Ensuring the quality of laboratory test results -- -- 1.6.
Recording, reporting and interpretation of results -- -- 2.
The Selection, Use And Maintenance Of Laboratory Equipment And Supplies -- -- 2.1.
Criteria for the selection of appropriate equipment --
Equipment maintenance, servicing and repairs -- -- 2.2.
Specific equipment --
Refrigerators and freezers --
Principles --
Care, maintenance and quality control --
Autoclaves --
Standard care, maintenance and quality control for a simple bench top autoclave --
Centrifuges --
Hand-operated centrifuge --
Microhaematocrit centrifuge --
General purpose centrifuge --
Care, maintenance and general operating rules --
pH meters --
The principle of pH --
Simple methods of assessing pH --
Guidelines for use --
Incubators --
Care, maintenance and quality control --
Ovens --
Balances --
Use, maintenance and quality control --
Water baths --
Use and maintenance of water baths --
Water distillers and deionisers --
Distillers --
Use, care and maintenance --
Deionisers --
Automatic/calibrated pipettes and dispensers --
Pipettes --
How to use a `displacement' micropipettor --
Dispensers --
Colorimetery equipment and spectrophotometers --
Spectrophotometers --
Use and care of a colorimeter/spectrophotometer --
Microscopes --
The compound microscope --
Parts of the microscope --
Precautions --
Using the light microscope --
Dark field illumination --
How to get the most from your microscope --
The microscopic field --
Systematic examination --
Calibration of the microscope --
Using a micrometer-eyepiece and a counting chamber to measure the actual size of objects -- -- 2.3.
A note on ELISA tests and specialized technology --
ELISA plate reader --
Polymerase chain reaction (PCR) --
Electron microscopy (EM) --
Automated haematology and biochemistry systems -- -- 2.4.
General laboratory ware --
Glassware and plastic --
Bottles --
Beakers --
Flasks --
Graduated cylinders --
Pipettes --
Care and cleaning of laboratory ware --
Cleaning and disinfection procedures are outlined below --
Use of detergents for cleaning glassware --
Use of ultrasonic baths --
Physical methods of sterilization --
Radiation --
Filtration --
Chemical methods of sterilization --
Phenolics -- -- 3.
Parasitology -- -- 3.1.
Introduction -- -- 3.2.
General helminthology --
Collection and preservation of faecal samples --
Methods used to look for helminth eggs in faeces --
Preparation of a standard suspension --
Qualitative techniques --
Quantitative techniques --
Modified Stoll counting technique (sedimentation) --
Culture, recovery and identification of larvae --
Larval extraction for lungworm larvae --
Larval culture --
Other helminthological techniques --
Post-mortem (necropsy) --
Total worm count --
Interpretation of parasitology results -- -- 3.3.
Helminths of veterinary importance --
Nematodes (roundworms) --
Cestodes --
Trematodes (flukes) --
Effects of parasitic helminths on their hosts --
Diseases caused by nematodes (roundworms) --
Gastrointestinal nematodes --
A note on anthelmintic resistance --
Specific nematodes of veterinary significance --
Nematodes found in the `stomach' or abomasum --
Nematodes found in the small intestine --
Nematodes found in the large intestine and caecum --
Large `roundworms' or ascarids --
Disease process and clinical signs --
Nematodes which live in the lungs --
Nematodes which live in other organs --
Diseases caused by cestodes --
Taenia sp. tapeworms (Cysticercosis) --
Coenurosis or Gid --
Echinococcosis or Hydatidosis --
Diseases caused by trematodes --
Fluke (Fascioliasis) --
Stomach fluke or conical fluke infection (Paramphistomum sp.) --
Dicrocoeliosis (Dicrocoelium dendriticum) --
Schistosomiasis (Schistosoma sp.) --
PROTOZOA -- -- 3.4.
Introduction to protozoal organisms --
Classification, development and life cycles -- -- 3.5.
Protozoal diseases --
Coccidiosis --
Prevention --
Treatment --
Toxoplasmosis --
Diagnosis --
Prevention and control --
Sarcocystis --
Diagnosis --
Prevention and control --
Neospora caninum --
Cryptosporidia --
Diagnosis --
Prevention and control --
Trypanosomiasis and leishmaniasis --
Other flagellates --
Other protozoa --
Balantidiasis -- -- 3.6.
Haemoparasites of ruminants --
Diagnosis, control and prevention --
Preparation and examination of blood and tissue smears --
Methods --
Smear preparation --
Stains and staining --
Stain preparation --
Concentrating Microfilariae and Trypanosomes --
Immunological tests --
Diseases caused by haemoparasites in ruminants --
Theileriasis --
Life cycle --
Clinical disease --
Diagnosis --
Control and prevention --
Babesiosis (Piroplasmosis) = red water fever --
Anaplasmosis-Gall sickness --
Trypanosomiasis --
Other trypanosomes --
The `One Health' approach -- -- 3.7.
Introduction to veterinary entomology --
Classification and identification --
Samples for ectoparasite examination -- -- 3.8.
Ticks and tick borne diseases --
Tick identification --
Prevention and control -- -- 3.9.
Mites and mange --
Skin scrapings and tissue digestion --
Examination procedure -- -- 3.10.
Lice and other insects --
Lice --
Lice common in livestock --
Anoplura (sucking lice) --
Mallophaga (biting lice) --
Other insects --
Identification of insects --
Diseases transmitted by arthropod vectors -- -- 4.
Microbiology -- -- 4.1.
General sample collection, preparation and handling -- -- 4.2.
Introduction to bacteriology --
Anatomy of the bacterial cell --
Brief description of cell components and their function --
Cell wall --
Cytoplasmic membrane --
Cytoplasm --
Genetic information --
Capsule --
Flagella --
Fimbriae or pili --
Endospores --
Morphology and classification -- -- 4.3.
Sample collection, preparation and submission --
Samples commonly collected --
Laboratory examination of specimens --
Routine and special stains used for microbiology smears --
Gram stain --
Modified Ziehl Neelsen Stain -- -- 4.4.
Culture and identification of bacteria --
Media and special requirements --
Oxygen requirements --
Media --
Sterility --
Storage --
General types of media and terminology used --
pH requirements --
Temperature --
Routine approach to samples submitted for bacteriological examination --
Goals of primary inoculation --
Identification of bacteria --
Routine stains used in microbiology --
The Gram stain --
Biochemical tests --
Beta-galactosidase test --
Oxidation fermentation test --
Catalase test --
Citrate test --
Coagulase test --
Gelatine liquefaction --
Hydrogen sulphide production --
Methyl red (MR) reaction --
Voges-Proskauer (VP) reaction --
Nitrate reduction test --
Oxidase (cytochrome oxidase) test --
Urea hydrolysis test --
Motility test --
Commercial identification systems for bacteria --
Serological typing --
Quantitative tests-counting bacteria -- -- 4.5.
Antibiotic sensitivity and antibiotic use --
Disk-diffusion antibiotic sensitivity tests --
Choice of drug for disc (disk) test --
Examples of suitable `first-line' test antibiotics --
Culture medium --
Test procedure --
Inoculation of primary cultures direct from specimen --
From pure sub-culture --
Control cultures --
Reading and interpretation of results --
A note on antimicrobial resistance --
Acquired resistance --
Multiple resistance --
Epidemiology of resistance --
How to select an antimicrobial drug --
Accurate diagnosis --
Antibiotic sensitivity testing --
Treatment selection and efficacy --
Duration and feasibility --
Drug withholding period --
Alternatives to antibiotics -- -- 4.6.
Mycology --
Moulds and yeast --
Clinical importance --
Laboratory diagnosis --
Fungal infections of the skin --
Specimens to collect --
Examples of specific fungal diseases --
Ringworm --
Direct microscopy --
Appearance of skin scales, nails or crusts in KOH preparation --
Culture --
Choice of media
Note continued:
Fungi which are commonly isolated from cases of ringworm and other skin conditions in animals --
Other fungal pathogens --
Mycotoxins -- -- 4.7.
Virology --
Properties of viruses --
Methods used to diagnose viral infections --
Light microscope --
Electron microscope --
Fluorescence --
Viral culture methods --
Laboratory animals --
Cell culture --
Virus propagation in chicken embryos --
Techniques of embryo inoculation --
Methods used to diagnose viral diseases --
Agglutination and precipitation --
Compliment fixation --
Neutralisation tests --
Virus haemagglutination (HA test) --
Haemagglutination-inhibition (HI) --
ELISA -- serology and antigen capture --
Protection tests --
Benefits and limitations of rapid `pen side' tests -- -- 4.8.
Molecular microbiology and its application as a diagnostic tool --
Introduction --
Techniques --
Hybridisation --
DNA:DNA/DNA:RNA --
Probes and microarrays --
DNA restriction --
Pulsed Field Gel Electrophoresis (PFGE) --
Amplification --
Conventional PCR --
Real time PCR (qPCR) --
Binary Typing using PCR --
Isothermal amplification --
Sequencing --
Multi Locus Sequence Typing (MLST) --
Digitalised isolate information, bioinformatics and epidemiology --
Next generation sequencing and metagenomics -- -- 5.
Haematology -- -- 5.1.
Introduction --
The haematopoietic system: terminology --
Blood volume and the appearance and characteristics of blood cells -- -- 5.2.
Collection of specimens --
Preparation and staining of blood and bone marrow smears --
Method --
Stain preparation -- -- 5.3.
Cell counts and white cell indices --
Peroxidase reaction --
Differential white blood cell count --
Total cell counts --
Total white cell count --
Calculation (depends on the type of haemocytometer used) --
Total red cell count --
Calculation (depends on the type of haemocytometer used) -- -- 5.4.
Red cell indices and anaemia --
Anaemia --
Packed cell volume (PCV) --
Determination of haemoglobin content --
Sahli's acid haematin method --
Method --
Cyanmethaemoglobin method --
Erythrocyte sedimentation rate --
Method --
Interpretation (simplified) -- -- 5.5.
Collection and examination of smears from bone marrow -- -- 5.6.
Determination of normal values -- -- 5.7.
Interpretation of results -- -- 5.8.
A note on automated systems -- -- 6.
Serology And Immunology -- -- 6.1.
Introduction --
The immune response -- -- 6.2.
Antigens and antibodies --
Antigen --
Antibody/immunoglobulins --
Structure and function of immunoglobulins (ig) --
Primary antibody response --
Definition and use of terms -- -- 6.3.
Tests used to measure antibody/antigen reactions --
Agglutination and precipitation tests --
Slide agglutination --
Equipment --
Procedure --
Recording the results --
Tube agglutination --
Example of the tube agglutination test --
Equipment --
Method --
Results --
Interpretation --
Immunodiffusion tests --
Haemagglutination tests --
Haemagglutination inhibition test --
HA test --
Titration of virus by haemagglutination --
Haemagglutination inhibition test (HI test) --
Haemagglutination Inhibition test --
Reagents --
Complement fixation test --
Fluorescent antibody tests --
Enztyme linked immunosorbent assay (ELISA) --
The Direct ELISA --
The indirect ELISA -- -- 6.4.
Quality control and interpretation of results -- -- 6.5.
Epidemiology and sampling plans -- -- 7.
Clinical Chemistry -- -- 7.1.
Introduction -- -- 7.2.
Blood sample collection and handling --
Sample collection --
Serum vs plasma --
Transport and processing --
Storage and preservation -- -- 7.3.
General health assessment --
Simple dip-stick tests --
Photometer methods -- -- 7.4.
Colorimetry explained --
The scientific principles --
Colour and light absorption --
Practical considerations --
Quality control -- -- 7.5.
Interpretation of clinical chemistry results -- -- 7.6.
General principles for basic biochemical tests --
Principles of chemical reactions --
Oxidation and reduction --
Electrolytes and electrolysis --
Acids and bases --
Buffers --
Indicators --
Techniques for preparing solutions --
Practical points to consider for accurate preparation of solutions --
Expressing the concentration of solutions --
Preparation of molar (mol/l) solutions and dilutions --
Preparing dilutions --
Saturated solutions --
Diluting techniques --
Examples of simple qualitative tests for district laboratories -- -- 1.
Iodine flocculation test for liver function -- -- 2.
Rapid Spot test for blood ketones -- -- 3.
Estimation of serum bilirubin -- -- 4.
Serum total protein by the Biuret method -- -- 5.
Haemoglobin determination -- -- 7.7.
Metabolic diseases and endocrine disorders -- -- 7.8.
Mineral and trace element assays -- -- 7.9.
Urinalysis --
Biochemical analysis of urine --
Microscopic examination of urine sediment --
Method --
Interpretation --
Looking at a centrifuged deposit --
Examining a centrifuged deposit --
Interpretation of results --
Biochemical tests --
Casts and cells -- -- 7.10.
Toxicology -- -- 8.
PATHOLOGY/CYTOLOGY -- -- 8.1.
Introduction -- -- 8.2.
General approach for necropsy cases --
Taking a case history and clinical examination --
Basic post-mortem equipment for field work --
Sample collection (equipment required) --
Brief guidelines for sample collection --
Procedure for post-mortem examinations --
Performing the necropsy --
Examination of specific body systems --
Gastrointestinal system --
Cardiovascular system --
Respiratory system --
Urogenital system --
Lymphoreticular system --
Neurological system --
Musculoskeletal system --
Endocrine system -- -- 8.3.
Histopathology --
Tissues from post-mortem cases --
Tissues from biopsy samples -- -- 8.4.
Cytology -- -- 8.5.
Examination of samples and interpretation of results --
Interpretation -- -- 9.
SPECIAL TOPICS -- -- 9.1.
Introduction to epidemiology -- -- 9.2.
Disease surveillance systems; resources and reporting requirements --
Background --
The role of veterinary para-professionals in disease surveillance -- -- 9.3.
Common clinical problems --
Infertility and abortion --
Infertility in the female --
Abortion --
History --
Clinical examination of dam and sire (if natural service) --
Examination of the placenta and foetus --
Laboratory samples --
Diarrhoea --
Take a full history --
Perform a thorough clinical examination --
Collect laboratory samples --
Haematuria and haemoglobinuria (red urine) --
Clinical history --
Clinical examination --
Laboratory samples --
Hair loss and itchy skin --
Ill thrift and chronic weight loss --
Neurologi signs -- -- 9.4.
Wildlife hea monitoring and disease surveillance --
Wildlife health and Disease -- A Canadian Perspective --
Thinking side the Box --
A Can Example: Caribou Health Monitoring -- -- 9.5.
Arthropod rs and Arthropod Borne Diseases --
Monitori disease vectors --
Arthro monitoring --
Mosqi and other biting flies --
Ticks --
Molecular agnostics --
Ticks --
Special lerations in the diagnostics of arthropod borne viruses-Virolog --
Cell cult --
Molecular gnostics -- -- 10.
APPENDICE -- -- 10.1a.
Zoonotic di s (summary) -- -- 10.1b.
Zoonotic di in brief --
Disease: rax --
Disease: Brucellosis --
Disease: Leptospirosis --
Disease: Tuberculosis --
Disease: Meliodosis --
Disease: Salmonellosis --
Disease: Plague --
Disease: Typhus --
Disease: Rabies --
Disease: Japanese B encephalitis --
Disease: Ringworm --
Disease: Leishmaniasis --
Disease: Toxoplasmosis --
Disease: Trichinosis --
Disease: Visceral larva migrans --
Disease: Cutaneous larva migrans --
Disease: Hydatids --
Disease: Tapeworm --
Disease: Schistosomiasis -- -- 10.2.
Post mortem and sample submission forms -- -- 10.3.
Some micro-organisms commonly isolated from animals -- -- 10.4.
Examples of laboratory equipment and reagent suppliers.
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Includes bibliographical references and index.
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| July 31, 2019 | Edited by MARC Bot | associate edition with work OL12360727W |
| December 3, 2010 | Edited by Open Library Bot | Added subjects from MARC records. |
| April 28, 2010 | Edited by Open Library Bot | Linked existing covers to the work. |
| January 23, 2010 | Edited by WorkBot | add more information to works |
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