An edition of Cereal genomics (2014)

Cereal genomics

methods and protocols

by and

Cereal genomics
Robert J. Henry, Agnelo Furtad ...
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Last edited by MARC Bot
October 7, 2024 | History
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An edition of Cereal genomics (2014)

Cereal genomics

methods and protocols

by and

In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes. Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described. Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.

Publish Date
Publisher
Humana Press
Language
English
Pages
300

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Subjects

Grain, Laboratory manuals, Genetics, Cereals, Genetic Techniques, Plant Genome, Genomics, Methods, Gentechnologie, Getreide, Grain, breeding, Life sciences, Plant Sciences, Plant Genetics & Genomics, Transgenics, Breeding
Edition Availability
Cover of: Cereal Genomics
Cereal Genomics: Methods and Protocols
2016, Humana Press
in English
Cover of: Cereal genomics
Cereal genomics: methods and protocols
2014, Humana Press
in English

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Book Details

Table of Contents

DNA Extraction from Vegetative Tissue for Next Generation Sequencing?
DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples)
RNA Extraction from Cereal Vegetative Tissue
RNA Extraction from Developing or Mature Wheat Seeds
cDNA Library Preparation
Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants
The Polymerase Chain Reaction (PCR)
General Methods
Mutation and Mutation Screening
The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression
Cloning of DNA Fragments: Ligation Reactions in Agarose Gel
Rapid Cloning of Genes and Promoters for Functional Analyses
Genome Walking
Functional Analysis by Protein Biochemistry
Genomic Southern Blot analysis
Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs
Protein Blotting-Protocol for Beginners
Genetic Transformation of Wheat via Particle Bombardment
Sorghum Genetic Transformation by Particle Bombardment
Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery
A Protocol for High-throughput Agrobacterium-mediated Barley Transformation
Agrobacterium-mediated Transformation- Rice Transformation
Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos
A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops
A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data.

Edition Notes

Includes bibliographical references and index.

Published in
New York
Series
Methods in molecular biology -- 1099, Springer protocols, Methods in molecular biology (Clifton, N.J.) -- v. 1099., Springer protocols (Series)

Classifications

Dewey Decimal Class
633.1/0423
Library of Congress
SB189.53 .C47 2014, QH301-705, QK495.G74

The Physical Object

Pagination
x, 300 pages
Number of pages
300

ID Numbers

Open Library
OL31015095M
ISBN 10
1627037144
ISBN 13
9781627037143, 9781627037150
LCCN
2013953327
OCLC/WorldCat
857967196, 867545931

Source records

Library of Congress MARC record
Better World Books record
harvard_bibliographic_metadata record

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October 7, 2024 Edited by MARC Bot import existing book
October 5, 2021 Edited by ImportBot import existing book
November 12, 2020 Created by MARC Bot import new book