The role of T cells in the regulation of acrolein-induced pulmonary inflammation and epithelial-cell pathology

with a critique by the HEI Health Review Committee

The role of T cells in the regulation of acro ...
Health Effects Institute. Heal ...
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Last edited by MARC Bot
December 12, 2022 | History

The role of T cells in the regulation of acrolein-induced pulmonary inflammation and epithelial-cell pathology

with a critique by the HEI Health Review Committee

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Publish Date
Language
English
Pages
42

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Book Details


Table of Contents

Methods. Mice
Acrolein exposure
Bronchoalveolar lavage
Cell and differential counts
Tissue fixation, histology, and immunohistochemistry
Quantification of mucous-cell metaplasia
BAL mucin assay
Lung-cell isolation and flow cytometry
T-cell purification
RNA isolation and amplification
Experimental design of microarrays
Microarray hybridization
Microarray analysis
Quantitative real-time PCR
ELISA
Statistical analysis
Results. Acrolein-induced pulmonary pathology
Injury to BAL epithelial cells
Apoptosis of pulmonary epithelial cells
Pulmonary inflammation
Levels of macrophage mediators in the lung
Purification of pulmonary lymphocytes
Microarray data
Cytokines and chemokines
Discussion and conclusions. Effects of acrolein on pulmonary pathology
Roles of [alpha][beta] T cells and [gamma][delta] T cells
Transcriptional analysis of [alpha][beta] T cells and [gamma][delta] T cells.

Edition Notes

"December 2009."

"Walter A. Rosenblith New Investigator Award research report."

Includes bibliographical references.

Published in
Boston, MA
Series
Research report -- no. 146, Research report (Health Effects Institute) -- no. 146.

The Physical Object

Pagination
vii, 42 p.
Number of pages
42

ID Numbers

Open Library
OL43899787M
OCLC/WorldCat
555687580

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