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Subjects
Laboratory manuals, Molecular cloningEdition | Availability |
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Molecular cloning, the condensed protocols: a laboratory manual
2006, Cold Spring Harbor Laboratory Press
in English
0879697725 9780879697723
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Book Details
Table of Contents
Plasmids and their usefulness in molecular cloning
Bacteriophage and its vectors
Working with bacteriophage M13
Working with high-capacity vectors
Gas electrophoresisof DNA and pulsed-field agarose
Preparation and analysis of eukaryotic genomic DNA
Extraction, purification, and analysis of mRNA from eukaryotic cells
In vitro amplification of DNA by the polymerase chain reaction
Preparation of radiolabeled DNA and RNA probes
Working with synthetic oligonucleotide probes
Preparation of cDNA libraries and gene identification
DNA sequencing
Mutagenesis
Screening expression libraries
Expression of cloned genes in escherichia coli
Introducing cloned genes into cultured mammalian cells
Analysis of gene expression in cultured mammalian cells
Protein interaction technologies.
Edition Notes
Includes index.
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