An edition of Receptor signal transduction protocols
(2011)
Receptor signal transduction protocols
3rd ed.
by Gary B. Willars and R. A. J. Challiss
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Receptor Signal Transduction Protocols: Third Edition
2016, Humana Press
in English
1493956256 9781493956258
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Book Details
Table of Contents
Inducible expression of G protein-coupled receptors in transfected cells -- Beryl Koener and Emmanuel Hermans
Using the Flp-In T-Rex system to regulate GPCR expression -- Richard J. Ward, Elisa Alvarez-Curto, and Graeme Milligan
Viral infection for GPCR expression in eukaryotic cells -- Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi
Generation of epitope-tagged GPCRs -- Yan Huang and Gary B. Willars
Use of site-directed mutagenesis to study GPCRs -- Alex C. Conner ... [et al.]
Approaches to study GPCR regulation in native systems -- Jonathon M. Willets
Heterologous expression of GPCRs in fission yeast -- John Davey and Graham Ladds
Radioligand binding methods for membrane preparations and intact cells -- David B. Bylund and Myron L. Toews
Quantification of GPCR mRNA using real-time RT-PCR -- Trond Brattelid and Finn Olav Levy
Determining allosteric modulator mechanism of action : integration of radioligand binding and functional assay data -- Christopher J. Langmead
Design and use of fluorescent ligands to study ligand-receptor interactions in single living cells -- Stephen J. Briddon, Barrie Kellam, and Stephen J. Hill
Examining site-specific GPCR phosphorylation -- Adrian J. Butcher, Andrew B. Tobin, and Kok Choi Kong
Ubiquitination of GPCRs -- Adriana Caballero and Adriano Marchese
[35S]GTPgS binding as an index of total G-protein and Ga-subtype-specific activation by GPCRs -- Rajendra Mistry, Mark R. Dowling, and R. A. John Challiss
Using calcium imaging as a readout of GPCR activation -- Martin D. Bootman and H. Llewelyn Roderick
Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors -- Frank Gesellchen ... [et al.]
Determining the activation of Rho as an index of receptor coupling to G12/13 proteins -- Michio Nakaya ... [et al.]
Use of translocating fluorescent biosensors for real-time monitoring of GPCR-mediated signaling events -- Carl P. Nelson and R.A. John Challiss
Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs -- Ashley E. Brady ... [et al.]
Study of GPCR-protein interactions by BRET -- Martina Kocan and Kevin D.G. Pfleger
Time resolved FRET strategy with fluorescent ligands to analyze receptor interactions in native tissues : application to GPCR oligomerization -- Martin Cottet ... [et al.]
Peptide affinity purification for the isolation and identification of GPCR-associated protein complexes -- Pascal Maurice, Avais M. Daulat, and Ralf Jockers
Tandem affinity purification and identification of GPCR-associated protein complexes -- Avais M. Daulat, Pascal Maurice, and Ralf Jockers
Identification of GPCR localization in detergent resistant membranes -- Ranju Kumari and Anna Francesconi
Analysis of GPCR localization and trafficking -- James N. Hislop and Mark von Zastrow
Statistical methods in research -- Domenico Spina.
Edition Notes
Includes bibliographical references and index.
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| August 17, 2023 | Edited by ImportBot | import existing book |
| October 17, 2020 | Edited by MARC Bot | import existing book |
| December 31, 2011 | Created by LC Bot | import new book |